Sharma Arti, Verma Pramod Kumar, Dixit V P
Department of Zoology, Raj Rishi College, Alwar (Raj) 301001, India.
Asian J Androl. 2003 Jun;5(2):121-4.
To evaluate the effect of an ethanolic extract of Semecarpus anacardium fruits on spermatogenesis in albino rats.
Male albino rats were fed with a 50 % ethanolic extract of Semecarpus anacardium fruit at 100 mg.kg(-1).day(-1), 200 mg.kg(-1).day(-1) and 300 mg.kg(-1).day(-1) for 60 days. Fertility test was performed after 60 days of treatment. Sperm motility and density were observed in the cauda epididymis. Biochemical and histological analyses of the blood and reproductive organs were done. Recovery of fertility was followed to evaluate the reversibility of drug action.
S. anacardium fruit extract administration resulted in spermatogenic arrest in albino rats. The sperm motility and density was reduced significantly. The RBC and WBC counts, haemoglobin, haematocrit, blood sugar and urea were found to be within the normal range in the whole blood. The protein, cholesterol and glycogen in the testes and the fructose in the seminal vesicle were significantly decreased after the treatment. The fruit extract feeding caused marked reduction in the number of primary spermatocytes, secondary spermatocytes and spermatids. The number of mature Leydig cells was also decreased and degenerating cells increased proportionately.
S. anacardium fruit extract causes spermatogenic arrest in albino rats.
评估印度楝果实乙醇提取物对白化大鼠精子发生的影响。
雄性白化大鼠分别以100 mg.kg⁻¹.day⁻¹、200 mg.kg⁻¹.day⁻¹和300 mg.kg⁻¹.day⁻¹的剂量喂食印度楝果实50%乙醇提取物，持续60天。治疗60天后进行生育力测试。观察附睾尾部的精子活力和密度。对血液和生殖器官进行生化和组织学分析。跟踪生育力恢复情况以评估药物作用的可逆性。
给予印度楝果实提取物导致白化大鼠精子发生停滞。精子活力和密度显著降低。全血中的红细胞和白细胞计数、血红蛋白、血细胞比容、血糖和尿素均在正常范围内。治疗后，睾丸中的蛋白质、胆固醇和糖原以及精囊中的果糖显著减少。喂食果实提取物导致初级精母细胞、次级精母细胞和精子细胞数量明显减少。成熟的间质细胞数量也减少，退化细胞相应增加。
印度楝果实提取物导致白化大鼠精子发生停滞。
