Cao Li-zhen, Lin Zhi-bin
Department of Pharmacology, School of Basic Medical Sciences, Health Science Center, Peking University, Beijing 100083, China.
Yao Xue Xue Bao. 2003 Feb;38(2):92-7.
To compare the influences of wood-cultured Ganoderma lucidum polysaccharides (Gl-PS-WC) and bag-cultured Ganoderma lucidum polysaccharides (Gl-PS-BC) on the proliferation activities of murine spleen lymphocytes in vitro, and investigate whether Gl-PS-BC can be substituted for Gl-PS-WC.
Mixed lymphocyte culture (MLC) reaction, lymphocyte proliferation induced by concanavalin A (Con A, 1 mg.L-1) or lipopolysaccharide (LPS, 5 mg.L-1), MLC reactions inhibited by immunosuppressive drugs, cyclosporine A (CsA, 0.1 mg.L-1), mitomycin (Mit C, 0.1 mg.L-1), or antitumor drug, etoposide (VP-16, 0.1 mg.L-1), were detected in the presence or absence of Gl-PS-WC and Gl-PS-BC in the concentration range of 0.2-12.8 mg.L-1.
Two kinds of polysaccharides were shown to promote MLC in the range of 0.2-12.8 mg.L-1, increase lymphocyte proliferation induced by Con A or LPS and antagonize the inhibitory effects of CsA, Mit C or VP-16 on MLC. No significant difference was observed between these two kinds of polysaccharides in selected concentrations.
Gl-PS-WC and Gl-PS-BC showed similar effects on the proliferation activities of murine spleen lymphocytes in vitro.
比较木生灵芝多糖（Gl-PS-WC）和袋栽灵芝多糖（Gl-PS-BC）对小鼠脾淋巴细胞体外增殖活性的影响，并探讨Gl-PS-BC是否可替代Gl-PS-WC。
在存在或不存在浓度范围为0.2-12.8mg.L-1的Gl-PS-WC和Gl-PS-BC的情况下，检测混合淋巴细胞培养（MLC）反应、伴刀豆球蛋白A（Con A，1mg.L-1）或脂多糖（LPS，5mg.L-1）诱导的淋巴细胞增殖、免疫抑制药物环孢素A（CsA，0.1mg.L-1）、丝裂霉素（Mit C，0.1mg.L-1）或抗肿瘤药物依托泊苷（VP-16，0.1mg.L-1）对MLC反应的抑制作用。
两种多糖在0.2-12.8mg.L-1范围内均能促进MLC，增加Con A或LPS诱导的淋巴细胞增殖，并拮抗CsA、Mit C或VP-16对MLC的抑制作用。在选定浓度下，这两种多糖之间未观察到显著差异。
Gl-PS-WC和Gl-PS-BC对小鼠脾淋巴细胞体外增殖活性显示出相似的作用。
