Wang Peng-yun, Wang Sai-zhen, Lin Shu-qian, Lin Zhi-bin
Department of Pharmacology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100083, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2005 Dec 18;37(6):569-74.
To compare the immunomodulatory effects of spore polysaccharides (Gl-SP) and broken spore polysaccharides (Gl-BSP) isolated from Ganoderma lucidum(Leyss et Fr.) Karst. on murine splenic lymphocytes and peritoneal macrophages in vitro.
Mixed lymphocyte culture reaction (MLR), lymphocyte proliferation in the presence or absence of mitogen, and the cytotoxic activity of splenic natural killer (NK) cells were detected with MTT assay in vitro. The percentage of phagocytosis of neutral red (NR) by mouse peritoneal macrophages was detected by colorimetric assay. Splenic T-lymphocyte subpopulations were measured with flow cytometry(FCM). IL-2, IFN-gamma and TNF-alpha in the culture supernatants were detected by ELISA and biological assay. Nitric oxide (NO) production was examined by Griess reaction.
At the concentration range of 0.2-12.8 mg/L, Gl-SP and Gl-BSP were shown to increase lymphocyte proliferation in the presence or absence of mitogen, enhance NK cytotoxic activity, augment the production of TNF-alpha and NO in Gl-SP- or Gl-BSP-activated macrophages, as well the percentage of phagocytosis of NR by macrophages in vitro. Both Gl-SP and Gl-BSP could promote MLR, however, at the dose of 12.8 mg/L, Gl-BSP showed higher activity than Gl-SP in the proliferation of lymphocytes. These two kinds of polysaccharide could significantly increase the secretion of IL-2 and IFN-gamma in doublejway MLR at the concentrations of 0.2-12.8 mg/L, but Gl-BSP had stronger effects than Gl-SP at the same concentrations. Both Gl-SP and Gl-BSP could increase the ratio of T-lymphocyte subpopulations in double-way MLR. At the concentrations of 0.2-12.8 mg/L or 3.2-12.8 mg/L, Gl-BSP demonstrated more significant activity in increasing the percentage of the CD4(+) or CD8(+) subset than Gl-SP. At the concentrations of 0.2-0.8 mg/L, the ratio of the CD4(+) and CD8(+) subset in the Gl-BSP treated group was higher than that of the Gl-SP treated group.
Gl-SP and Gl-BSP have similar immunomodulatory effects in vitro, as though the immunomodulatory effects of Gl-BSP are stronger than that of Gl-SP.
比较从灵芝（Leyss et Fr.）Karst.中分离得到的孢子多糖（Gl-SP）和破壁孢子多糖（Gl-BSP）对小鼠脾淋巴细胞和腹腔巨噬细胞的体外免疫调节作用。
采用MTT法体外检测混合淋巴细胞培养反应（MLR）、有无丝裂原存在时淋巴细胞的增殖以及脾自然杀伤（NK）细胞的细胞毒活性。采用比色法检测小鼠腹腔巨噬细胞对中性红（NR）的吞噬百分率。用流式细胞术（FCM）检测脾T淋巴细胞亚群。通过ELISA和生物学检测法检测培养上清液中的白细胞介素-2（IL-2）、γ干扰素（IFN-γ）和肿瘤坏死因子-α（TNF-α）。通过Griess反应检测一氧化氮（NO）的产生。
在0.2 - 12.8 mg/L浓度范围内，Gl-SP和Gl-BSP在有无丝裂原存在时均可增加淋巴细胞增殖，增强NK细胞毒活性，增加Gl-SP或Gl-BSP激活的巨噬细胞中TNF-α和NO的产生，以及体外巨噬细胞对NR的吞噬百分率。Gl-SP和Gl-BSP均可促进MLR，然而，在12.8 mg/L剂量时，Gl-BSP在淋巴细胞增殖方面显示出比Gl-SP更高的活性。在0.2 - 12.8 mg/L浓度下，这两种多糖在双向MLR中均可显著增加IL-2和IFN-γ的分泌，但在相同浓度下Gl-BSP的作用强于Gl-SP。Gl-SP和Gl-BSP在双向MLR中均可增加T淋巴细胞亚群的比例。在0.2 - 12.8 mg/L或3.2 - 12.8 mg/L浓度下，Gl-BSP在增加CD4(+)或CD8(+)亚群百分率方面显示出比Gl-SP更显著的活性。在0.2 - 0.8 mg/L浓度下，Gl-BSP处理组中CD4(+)与CD8(+)亚群的比例高于Gl-SP处理组。
Gl-SP和Gl-BSP在体外具有相似的免疫调节作用，尽管Gl-BSP的免疫调节作用强于Gl-SP。
