Garcia M C, Ginther O J
Endocrinology. 1976 Apr;98(4):958-62. doi: 10.1210/endo-98-4-958.
Six pony mares were ovariectomized (OVX) on day 16 of diestrus during June and July, 1972, to study short term changes in plasma luteinizing hormone (LH) concentrations. Plasma LH was higher (P less than .05) 3 days after OVX (1.76 ng/ml) than the day after OVX (1.01 ng/ml), and a gradual increase occurred over the first 2 weeks. Elevated plasma LH concentrations similar to mid-estrus levels were present from the 2nd to 11th week post-OVX. In another experiment, the same 6 OVX mares were bled once a month from February, 1973, to January, 1974, to study long-term changes in plasma LH in relation to the anestrous (November-April) and breeding seasons (May-October). Mean monthly plasma LH concentrations in OVX mares during all months of the anestrous season were lower (P less than .05) than mean monthly plasma LH during the months of June-September of the breeding season. During the transition from anestrous to breeding seasons (April, May) and breeding to anestrous seasons October, November), mean plasma LH concentrations were not significantly different between April and May (1.39 vs 1.84 ng/ml) and between October and November (3.02 vs 2.56 ng/ml), but April and May levels were lower (P less than .05) than October and November levels. Mean monthly plasma LH levels in OVX mares during the anestrous season were similar to those in intact mares during the anestrous season and at mid-diestrus during the breeding season. Mean monthly plasma LH levels in OVX mares during the breeding season were similar to plasma LH levels at mild-estrus in intact mares. These data indicate the presence of inhibitory (most likely progesterone) and stimulatory (most likely estrogen) influences from the ovary which modulate the endogenous LH pattern in mares during the breeding season.
1972年6月和7月间，在发情后期的第16天，对6匹小母马进行了卵巢切除手术（OVX），以研究血浆促黄体生成素（LH）浓度的短期变化。卵巢切除术后3天（1.76纳克/毫升）的血浆LH水平高于术后第1天（1.01纳克/毫升）（P<0.05），并且在最初的2周内逐渐升高。卵巢切除术后第2周至第11周，血浆LH浓度升高至与发情中期水平相似。在另一项实验中，从1973年2月至1974年1月，每月对同样6匹接受卵巢切除术的母马采血一次，以研究与乏情期（11月至4月）和繁殖季节（5月至10月）相关的血浆LH的长期变化。在乏情季节的所有月份中，接受卵巢切除术的母马的平均每月血浆LH浓度低于繁殖季节6月至9月的平均每月血浆LH浓度（P<0.05）。在从乏情期过渡到繁殖季节（4月、5月）以及从繁殖季节过渡到乏情季节（10月、11月）期间，4月和5月之间（1.39对1.84纳克/毫升）以及10月和11月之间（3.02对2.56纳克/毫升）的平均血浆LH浓度没有显著差异，但4月和5月的水平低于10月和11月的水平（P<0.05）。接受卵巢切除术的母马在乏情季节的平均每月血浆LH水平与完整母马在乏情季节以及繁殖季节发情后期中期的水平相似。接受卵巢切除术的母马在繁殖季节的平均每月血浆LH水平与完整母马在轻度发情期的血浆LH水平相似。这些数据表明，卵巢存在抑制性（最可能是孕酮）和刺激性（最可能是雌激素）影响，这些影响在繁殖季节调节母马体内LH的模式。
