Raulais D, Hagaman J, Ontjes D A, Lundblad R L, Kingdon H S
Eur J Biochem. 1976 May 1;64(2):607-11. doi: 10.1111/j.1432-1033.1976.tb10340.x.
The complete amino acid sequence of rat thyrocalcitonin has been determined by automated Edman degradations of the intact molecule, a cyanogen bromide fragment, and by degradations of mixtures of peptides produced by hydrolysis of the hormone with trypsin and chymotrypsin. The sequence determined was H2N-Cys-Gly-Asn-Leu-Ser-Thr-Cys-Met-Leu-Gly-Thr-Tyr-Thr-Gln-Asp-Leu-Asn-Lys-Phe-His-Thr-Phe-Pro-Gln-Thr-Ser-Ile-Gly-Val-Gly-Ala-Pro-NH2. This sequence differs in only two positions from that found in the human hormone, i.e. leucine-16 in the rat vs phenylalanine-16 in the human, and serine-26 in the rat vs alanine-26 in the human. These similarities and differences are consistent with the previously reported immunological properties of the hormones isolated from these two species.
通过对完整分子、溴化氰片段进行自动埃德曼降解，并对用胰蛋白酶和糜蛋白酶水解该激素产生的肽混合物进行降解，确定了大鼠甲状腺降钙素的完整氨基酸序列。所确定的序列为H2N-Cys-Gly-Asn-Leu-Ser-Thr-Cys-Met-Leu-Gly-Thr-Tyr-Thr-Gln-Asp-Leu-Asn-Lys-Phe-His-Thr-Phe-Pro-Gln-Thr-Ser-Ile-Gly-Val-Gly-Ala-Pro-NH2。该序列与人类激素序列仅在两个位置上不同，即大鼠的第16位是亮氨酸，而人类的是苯丙氨酸；大鼠的第26位是丝氨酸，而人类的是丙氨酸。这些异同与先前报道的从这两个物种中分离出的激素的免疫学特性一致。
