Kazama Tomohiko, Toriyama Kinya
Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan.
FEBS Lett. 2003 Jun 5;544(1-3):99-102. doi: 10.1016/s0014-5793(03)00480-0.
A fertility restorer gene (Rf-1) of [ms-bo] cytoplasmic male sterility (CMS) in rice has been reported to be responsible for the processing of RNA of aberrant atp6 of mitochondria. We have carried out map-based cloning of the Rf-1 gene and found that a 4.7-kb genomic fragment of a restorer line promoted the processing of aberrant atp6 RNA when introduced into a CMS line. The genomic fragment contained a single open reading frame encoding 18 repeats of the 35 amino acid pentatricopeptide repeat (PPR) motif. The cloned PPR gene is a possible candidate of Rf-1. A non-restoring genotype was identified to have deletions within the coding region.
据报道，水稻[ms-bo]细胞质雄性不育（CMS）的育性恢复基因（Rf-1）负责线粒体异常atp6的RNA加工。我们对Rf-1基因进行了图位克隆，发现当将一个恢复系的4.7 kb基因组片段导入CMS系时，它能促进异常atp6 RNA的加工。该基因组片段包含一个单一的开放阅读框，编码18个35个氨基酸的五肽重复序列（PPR）基序。克隆的PPR基因可能是Rf-1的候选基因。已鉴定出一种非恢复基因型在编码区内存在缺失。
