Hoppe P C
J Reprod Fertil. 1975 Oct;45(1):109-15. doi: 10.1530/jrf.0.0450109.
Secretion of the pregnancy-blocking pheromone was stimulated by injection of depo-testosterone cypionate into females and males of inbred strains of mice which do not normally secrete the pheromone. Testosterone treatment of SJL males altered pheromone secretion so that pregnancies were blocked when the stud male was of the same inbred strain; an event that does not normally occur. Injection of epiandrosterone, androstenedione, androsterone or testosterone significantly increased pheromone secretion in SJL females, but progesterone and dehydroepiandrosterone were ineffective. Kidney weights were significantly increased by administration of androgen metabolites and the possibility of the kidney being the site of pheromone synthesis is discussed. The preputial gland can be excluded as the site of pheromone synthesis since males which are hemizygous for the Tabby-J gene and have no preputial glands blocked pregnancies as effectively as their normal littermates. Preliminary results are also presented concerning the isolation of the pregnancy-blocking pheromone from urine. Urine was analysed by gas chromatography and a peak was observed whose concentration could be correlated with secretion of the pheromone, although the compound(s) has not been identified or tested for biological activity.
通过向通常不分泌这种妊娠阻断信息素的近交系小鼠的雌性和雄性体内注射环丙孕酮睾酮，刺激了该信息素的分泌。对SJL雄性小鼠进行睾酮处理会改变信息素的分泌，使得当种雄鼠与雌鼠为同一近交系时，妊娠会被阻断；而这一情况通常不会发生。向SJL雌性小鼠注射表雄酮、雄烯二酮、雄酮或睾酮会显著增加信息素的分泌，但孕酮和脱氢表雄酮则无效。给予雄激素代谢产物会使肾脏重量显著增加，并对肾脏作为信息素合成部位的可能性进行了讨论。由于对于Tabby-J基因半合子且没有包皮腺的雄性小鼠，其阻断妊娠的效果与正常同窝小鼠一样有效，因此可以排除包皮腺作为信息素合成部位。还给出了从尿液中分离妊娠阻断信息素的初步结果。通过气相色谱法对尿液进行分析，观察到一个峰，其浓度与信息素的分泌相关，尽管该化合物尚未被鉴定或进行生物活性测试。
