Hashimoto Masayuki, Kato Jun-ichi
Department of Biology, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan.
Biosci Biotechnol Biochem. 2003 Apr;67(4):919-22. doi: 10.1271/bbb.67.919.
We found that a carbonic anhydrase, YadF, is essential for cell growth in the absence of another carbonic anhydrase, CynT, in Escherichia coli. However, mutant strains lacking both of them grew at high CO2 concentrations (5%), where non-enzymatic mechanisms generate HCO3-. This suggests that these carbonic anhydrases are essential because they maintain HCO3- levels at ambient CO2 concentrations.
我们发现，在大肠杆菌中，当缺乏另一种碳酸酐酶CynT时，碳酸酐酶YadF对细胞生长至关重要。然而，同时缺失这两种酶的突变菌株在高二氧化碳浓度（5%）下能够生长，在这种情况下非酶机制会产生碳酸氢根离子。这表明这些碳酸酐酶至关重要，因为它们在环境二氧化碳浓度下维持着碳酸氢根离子的水平。
