人精浆中一种精子包被抗原的纯化与鉴定

Purification and characterization of a sperm-coating antigen from human seminal plasma.

作者信息

Li T S, Macajoux C E, Devanesan M M, Pelosi M A, Caterini H, Kaminetzky H A

出版信息

Fertil Steril. 1976 Jun;27(6):702-12. doi: 10.1016/s0015-0282(16)41903-5.

DOI:10.1016/s0015-0282(16)41903-5

Abstract

A sperm-coating antigen has been purified from human seminal plasma by a combination of fractionation methods including ammonium sulfate precipitation, Sephadex G-100 gel filtration, and diethylaminoethyl cellulose ion-exchange chromatography. The purified antigen was demonstrated to be immunologically identical sperm-coating antigen, not derived from blood serum but having immunologically cross-relating antigens in milk and saliva, and a glycoprotein with a molecular weight of approximately 33,000. The purpose of purifying this antigen was discussed.

摘要

通过包括硫酸铵沉淀、Sephadex G - 100凝胶过滤和二乙氨基乙基纤维素离子交换色谱在内的分级分离方法组合，已从人精浆中纯化出一种精子包被抗原。纯化后的抗原被证明在免疫学上与精子包被抗原相同，并非源自血清，但在牛奶和唾液中有免疫交叉相关抗原，是一种分子量约为33,000的糖蛋白。文中讨论了纯化该抗原的目的。