Saji F, Minagawa Y, Ohashi K, Negoro T, Tanizawa O
Am J Reprod Immunol Microbiol. 1986 Sep;12(1):13-6. doi: 10.1111/j.1600-0897.1986.tb00053.x.
In our previous paper, we identified a novel sperm-coating antigen with molecular weight of approximately 12,000 daltons that is highly specific to sperm, seminal plasma, and milk from the plasma membrane fraction of human spermatozoa by using rabbit antiseminal plasma antiserum. In the present study, this 12,000-daltons component, termed gp12, has been investigated for its tissue distribution and antigenic stability. The largest amounts of the antigen are found in seminal plasma, although individual variation is rather high. In seminal plasma, the gp12 molecule presents in a large form with a molecular weight of approximately 50,000 daltons. Its antigenicity is stable when treated with acid, alkali, heat, and various protein denaturants.
在我们之前的论文中，我们通过使用兔抗血清，从人精子质膜部分鉴定出一种分子量约为12,000道尔顿的新型精子包被抗原，该抗原对精子、精浆和乳汁具有高度特异性。在本研究中，对这种称为gp12的12,000道尔顿成分的组织分布和抗原稳定性进行了研究。尽管个体差异相当大，但在精浆中发现的抗原量最大。在精浆中，gp12分子以分子量约为50,000道尔顿的大形式存在。用酸、碱、热和各种蛋白质变性剂处理时，其抗原性稳定。
