Pope Lisa H, Bennink Martin L, Greve Jan
Department of Applied Physics and MESA Research Institute, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands.
J Muscle Res Cell Motil. 2002;23(5-6):397-407. doi: 10.1023/a:1023450204528.
Recently significant success has emerged from exciting research involving chromatin stretching using optical tweezers. These experiments, in which a single chromatin fibre is attached by one end to a micron-sized bead held in an optical trap and to a solid surface or second bead via the other end, allows manipulation and force detection at a single-molecule level. Through force-induced stretching of chromatin, mechanical properties, specific intermolecular bond strengths and DNA-protein association and dissociation kinetics have been determined. These studies will be extremely fruitful in terms of understanding the function of chromatin structure and its dynamics within the cell.
最近，利用光镊对染色质进行拉伸的激动人心的研究取得了重大成功。在这些实验中，单个染色质纤维的一端连接到光阱中捕获的微米级珠子上，另一端连接到固体表面或另一个珠子上，从而能够在单分子水平上进行操纵和力检测。通过对染色质进行力诱导拉伸，已经确定了其机械性能、特定分子间键的强度以及DNA-蛋白质的结合和解离动力学。就理解染色质结构在细胞内的功能及其动态变化而言，这些研究将极具成效。
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