单分子拉伸实验中DNA相互作用的热力学

Thermodynamics of DNA interactions from single molecule stretching experiments.

作者信息

Williams Mark C, Rouzina Ioulia, Bloomfield Victor A

机构信息

Department of Biochemistry, Molecular Biology, and Biophysics, 1479 Gortner Avenue, University of Minnesota, Saint Paul, Minnesota 55108, USA.

出版信息

Acc Chem Res. 2002 Mar;35(3):159-66. doi: 10.1021/ar010045k.

DOI:10.1021/ar010045k

PMID:11900519

Abstract

On the basis of our analysis of detailed measurements of the dependence of the overstretching transition of double-stranded DNA (dsDNA) on temperature, pH, and ionic strength, we have demonstrated that a model of force-induced melting accurately describes the thermodynamics of DNA overstretching. Measurements of this transition allow us to determine the stability of dsDNA and obtain information similar to that obtained in thermal melting studies. This single-molecule technique has the advantage that it can be used to measure DNA stability at any temperature. We discuss the use of this technique to study the nucleic acid chaperone activity of the HIV-1 nucleocapsid protein.

摘要

基于我们对双链DNA（dsDNA）过度拉伸转变对温度、pH值和离子强度依赖性的详细测量分析，我们证明了力诱导解链模型能够准确描述DNA过度拉伸的热力学过程。对这种转变的测量使我们能够确定dsDNA的稳定性，并获得与热解链研究中所获得的信息相似的信息。这种单分子技术的优势在于它可用于在任何温度下测量DNA的稳定性。我们讨论了使用该技术研究HIV-1核衣壳蛋白的核酸伴侣活性。