Kobayashi Hiroichi, Sagara Junji, Masumoto Junya, Kurita Hiroshi, Kurashina Kenji, Taniguchi Shuníichiro
Department of Dentistry and Oral Surgery, Research Center on Aging and Adaptation, Shinshu University School of Medicine, Asahi 3-11, Matsumoto 390-8621, Japan.
J Oral Pathol Med. 2003 Jul;32(6):344-9. doi: 10.1034/j.1600-0714.2003.00111.x.
Moesin, a member of ERM (ezrin/radixin/moesin) family, links actin filaments of cell surface structure to the cell membrane. The purpose of the study is to assess the shifts in cellular distribution of moesin in normal oral epithelium, oral epithelial dysplasia (OED), verrucous carcinoma (VC), and oral squamous cell carcinoma (OSCC).
The expression of moesin was evaluated immunohistochemically in paraffin-embedded tissues of 59 specimens of OSCC, 35 specimens of OED, 17 specimens of VC, and five specimens of normal oral epithelium.
In the normal oral epithelia, all specimens showed a pattern of membranous expression against the anti-moesin antibody in the basal layer cells. In the OED specimens, moesin was dominantly expressed in the cell membrane except for the cornified layer. In VC and OSCC specimens, almost the whole of the carcinoma cells were stained with anti-moesin antibody. However, in OSCC samples, moesin was markedly expressed increasingly in the cytoplasm and decreasingly in the cell membrane, as compared with OED and VC. In addition, there was a significant correlation between the pattern of moesin expression and tumor differentiation in OSCC.
Our results suggest that it is useful to detect the moesin expression as adjunct to screening mucosal lesions in the oral cavity.
膜突蛋白是ERM（埃兹蛋白/根蛋白/膜突蛋白）家族的成员之一，它将细胞表面结构的肌动蛋白丝与细胞膜相连。本研究的目的是评估膜突蛋白在正常口腔上皮、口腔上皮发育异常（OED）、疣状癌（VC）和口腔鳞状细胞癌（OSCC）中的细胞分布变化。
采用免疫组织化学方法评估59例OSCC标本、35例OED标本、17例VC标本和5例正常口腔上皮石蜡包埋组织中膜突蛋白的表达。
在正常口腔上皮中，所有标本的基底层细胞对抗膜突蛋白抗体均呈膜性表达模式。在OED标本中，除角化层外，膜突蛋白主要表达于细胞膜。在VC和OSCC标本中，几乎所有癌细胞均被抗膜突蛋白抗体染色。然而，与OED和VC相比，在OSCC标本中，膜突蛋白在细胞质中的表达明显增加，在细胞膜中的表达减少。此外，OSCC中膜突蛋白的表达模式与肿瘤分化之间存在显著相关性。
我们的结果表明，检测膜突蛋白的表达作为口腔黏膜病变筛查的辅助手段是有用的。
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