Department of Oral Pathology and Oral Microbiology, Vinayaka Mission's Sankarachariyar Dental College and Hospitals, VMRF (DU) Salem, Tamil Nadu, India.
Department of Periodontics, Vinayaka Mission's Sankarachariyar Dental College and Hospitals, VMRF (DU) Salem, Tamil Nadu, India.
J Cancer Res Ther. 2024 Jul 1;20(5):1376-1383. doi: 10.4103/jcrt.jcrt_2406_22. Epub 2023 Oct 7.
Oral squamous cell carcinoma (OSCC) is a serious health disease that can lead to a reduced quality of life or even death. It ranks sixth in terms of cancer expansion. It is one of India's primary causes of natural death. In OSCC such potentially malignant Disorders (PMDs) are precancerous lesions with such a high risk of progression. Tumor angiogenesis is a one of the basic biomarkers that may influence the proliferation of a precancerous lesion into the cancerous lesion. Tropomyosin receptor kinase B (TrkB), vascular endothelial growth factor (VEGF), and brain-derived neurotrophic factor (BDNF) also play important roles in carcinogenesis by promoting angiogenesis. The construction of new vessels of blood from existing vasculature is referred as angiogenesis.
To get deep insights of immunohistochemistry expression of VEGF, BDNF, and TRKB in oral epithelial dysplasia (OED), verrucous carcinoma (VC), and OSCC.
The study included 100 formalin-fixed paraffin-embedded tissue blocks from 20 cases of OED, 20 cases of VC, and 60 cases of OSCC [20 cases of well-differentiated oral squamous cell carcinoma (WD-OSCC), 20 cases of moderately differentiated oral squamous cell carcinoma (MD-OSCC), and 20 cases of poorly differentiated oral squamous cell carcinoma (PD-OSCC). The staining intensity and distribution of VEGF, BNDF, and TrkB were examined and statistically analyzed using analysis of variance (ANOVA), post hoc Bonferroni test, independent t-test, Pearson's Chi-square test, and Pearson's correlation coefficient test.
The immunoexpression of VEGF, BDNF, and TrkB was found to be elevated in the order of OEDs, VC, and OSCC. The percentage of positive was highest in PD-OSCC, followed by MD-OSCC and WD-OSCC.
Based on our findings, angiogenesis plays a significant role in tumor growth and metastasis. A substantial relationship was discovered between VEGF, BDNF, TrkB expression, and increases in vascularity throughout the transition from OEDs to VCs and OSCCs.
口腔鳞状细胞癌（OSCC）是一种严重的健康疾病，可导致生活质量下降，甚至死亡。它在癌症扩散方面排名第六，是印度主要的自然死亡原因之一。在 OSCC 中，这种潜在的恶性疾病（PMD）是一种癌前病变，具有很高的进展风险。肿瘤血管生成是影响癌前病变向癌变发展的基本生物标志物之一。原肌球蛋白受体激酶 B（TrkB）、血管内皮生长因子（VEGF）和脑源性神经营养因子（BDNF）也通过促进血管生成在致癌作用中发挥重要作用。从现有脉管系统中构建新的血管血液被称为血管生成。
深入了解口腔上皮异型增生（OED）、疣状癌（VC）和 OSCC 中 VEGF、BDNF 和 TRKB 的免疫组织化学表达。
该研究包括来自 20 例 OED、20 例 VC 和 60 例 OSCC（20 例分化良好的口腔鳞状细胞癌（WD-OSCC）、20 例中度分化的口腔鳞状细胞癌（MD-OSCC）和 20 例分化不良的口腔鳞状细胞癌（PD-OSCC）的 100 例福尔马林固定石蜡包埋组织块。使用方差分析（ANOVA）、事后 Bonferroni 检验、独立 t 检验、Pearson 卡方检验和 Pearson 相关系数检验对 VEGF、BNDF 和 TrkB 的染色强度和分布进行了检查和统计分析。
发现 VEGF、BDNF 和 TrkB 的免疫表达在 OED、VC 和 OSCC 的顺序中升高。PD-OSCC 的阳性率最高，其次是 MD-OSCC 和 WD-OSCC。
根据我们的发现，血管生成在肿瘤生长和转移中起着重要作用。在 OED 向 VC 和 OSCC 过渡过程中，VEGF、BDNF、TrkB 表达与血管密度增加之间存在显著相关性。
