Straathof A J J
Kluyver Laboratory for Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
Biotechnol Prog. 2003 May-Jun;19(3):755-62. doi: 10.1021/bp025750m.
When an industrial process is developed using the microbial transformation of a precursor into a desired chemical compound, high concentrations of substrate and product will be involved. These compounds may become toxic to the cells. In situ product removal (ISPR) may be carried out, using auxiliary phases such as extractants or adsorbents. Simultaneously, in situ substrate addition (ISSA) may be performed. It is shown that for uncharged substrates and products, the aqueous solubilities of substrate and product can be used to predict if ISPR might be required. When a particular auxiliary phase is selected and the distribution coefficients of substrate and product are known, it is possible to estimate a priori if this auxiliary phase might be good enough and how much of it might be needed for an efficient (fed-)batch biotransformation process. For biotransformation products of intermediate polarity (aqueous solubility of about 1-10 g/L) there seems to be a lack of extractants and adsorbents with the capacity to raise the product concentrations to commercially more interesting levels.
当利用微生物将前体转化为所需化合物来开发工业过程时，会涉及高浓度的底物和产物。这些化合物可能对细胞产生毒性。可以使用萃取剂或吸附剂等辅助相进行原位产物去除（ISPR）。同时，可以进行原位底物添加（ISSA）。结果表明，对于不带电荷的底物和产物，可以使用底物和产物的水溶性来预测是否需要ISPR。当选择了特定的辅助相且已知底物和产物的分配系数时，就可以预先估计该辅助相是否足够好，以及对于高效的（补料）分批生物转化过程可能需要多少辅助相。对于中等极性的生物转化产物（水溶性约为1 - 10 g/L），似乎缺乏能够将产物浓度提高到更具商业价值水平的萃取剂和吸附剂。
