Ishida B Y, Paigen B
Division of Research Medicine and Radiation Biophysics, Lawrence Berkeley Laboratory, Berkeley, CA 94720.
J Lipid Res. 1992 Jul;33(7):1073-8.
Silver-staining of immunoprecipitates extends the sensitivity of the radial immunodiffusion assay by tenfold. This modification permits the quantification of apolipoproteins A-I, A-II, C, and E at levels of 0.2-1.0 mg/dl in plasma samples at a sensitivity threshold of 10 ng. The silver-enhanced radial immunodiffusion method is readily adapted from the standard method, simple and inexpensive to perform, and does not require costly instrumentation. These advantages make the modified RID assay an attractive alternative to other forms of immunoassay.
免疫沉淀物的银染色将单向免疫扩散测定的灵敏度提高了十倍。这种改进使得能够在10 ng的灵敏度阈值下，对血浆样本中载脂蛋白A-I、A-II、C和E进行定量，浓度范围为0.2 - 1.0 mg/dl。银增强单向免疫扩散法很容易从标准方法改编而来，操作简单且成本低廉，不需要昂贵的仪器设备。这些优点使得改进后的RID测定法成为其他免疫测定形式的一个有吸引力的替代方法。
