[用放射免疫扩散法测定人血清载脂蛋白A II]

[Quantification of human serum apolipoprotein A II by radial immunodiffusion].

作者信息

Luo J, Zhang Z, Liu Y, Liu B

出版信息

Hua Xi Yi Ke Da Xue Xue Bao. 1990 Mar;21(1):96-9.

Abstract

A radial immunodiffusion assay (RID) for human serum apolipoprotein A II (apo A II) was developed. Antisera to human apoA II were raised in goats with purified apoA II. The antibody-gel plate was prepared with 1% agarose. 5 microliters of each sample was added in a well. Diffusion was performed at 25 degrees C for 48 h. The minimum measurable concentration of apoA II was 58ng. The standard curve with a working range of 17.3-69 mg/dl was plotted. The coefficients of variation of within and between plates were 1.5-4.6% and 5.6% respectively. The recovery of the assay was 90.3-102.0%. The concentrations of apoA II for healthy men and women in Chengdu area were 26.7 +/- 4.4 mg/dl (n = 45) and 26.6 +/- 4.6 mg/dl (n = 31) respectively.

摘要

建立了一种用于检测人血清载脂蛋白A II（apo A II）的单向免疫扩散试验（RID）。用纯化的apoA II在山羊体内制备抗人apoA II血清。用1%的琼脂糖制备抗体凝胶板。每个孔中加入5微升的每个样品。在25℃下进行扩散48小时。apoA II的最低可测浓度为58纳克。绘制了工作范围为17.3 - 69毫克/分升的标准曲线。板内和板间的变异系数分别为1.5 - 4.6%和5.6%。该检测方法的回收率为90.3 - 102.0%。成都地区健康男性和女性的apoA II浓度分别为26.7±4.4毫克/分升（n = 45）和26.6±4.6毫克/分升（n = 31）。