Douglas James T, Qian Lishi, Montoya Jaime C, Musser James M, Van Embden Jan D A, Van Soolingen Dick, Kremer Kristin
Department of Microbiology, University of Hawaii, Honolulu, Hawaii 96822, USA.
J Clin Microbiol. 2003 Jun;41(6):2723-6. doi: 10.1128/JCM.41.6.2723-2726.2003.
Forty-eight Mycobacterium tuberculosis strains were obtained from patients living in metropolitan Manila, Republic of the Philippines. Three molecular typing methods, IS6110 restriction fragment length polymorphism, spoligotyping, and DNA sequencing of the oxyR, gyrA, and katG loci, established that these strains have restricted diversity and are members of a related genetic group of organisms. Comparison of the DNA fingerprint patterns with those in international databases confirmed the uniqueness of this group of isolates, which we designate the Manila family of M. tuberculosis.
从菲律宾共和国马尼拉大都会的患者中获取了48株结核分枝杆菌菌株。三种分子分型方法,即IS6110限制性片段长度多态性分析、间隔寡核苷酸分型以及oxyR、gyrA和katG基因座的DNA测序,证实这些菌株具有有限的多样性,并且属于相关遗传生物群体的成员。将这些DNA指纹图谱与国际数据库中的图谱进行比较,证实了这组分离株的独特性,我们将其命名为结核分枝杆菌马尼拉家族。