Alves Margarida, Xiao Lihua, Sulaiman Irshad, Lal Altaf A, Matos Olga, Antunes Francisco
Unidade de Protozoários Oportunistas/VIH e Outras Protozooses, Unidade de Parasitologia e Microbiologia Médicas (UPMM), Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, 1349-008 Lisbon, Portugal.
J Clin Microbiol. 2003 Jun;41(6):2744-7. doi: 10.1128/JCM.41.6.2744-2747.2003.
Cryptosporidium parvum and Cryptosporidium hominis isolates from human immunodeficiency virus-infected patients, cattle, and wild ruminants were characterized by PCR and DNA sequencing analysis of the 60-kDa glycoprotein gene. Seven alleles were identified, three corresponding to C. hominis and four corresponding to C. parvum. One new allele was found (IId), and one (IIb) had only been found in Portugal. Isolates from cattle and wild ruminants clustered in two alleles. In contrast, human isolates clustered in seven alleles, showing extensive allelic diversity.
通过对60 kDa糖蛋白基因进行PCR和DNA测序分析，对来自人类免疫缺陷病毒感染患者、牛和野生反刍动物的微小隐孢子虫和人隐孢子虫分离株进行了特征鉴定。共鉴定出7个等位基因，其中3个与人隐孢子虫对应，4个与微小隐孢子虫对应。发现了一个新的等位基因（IId），还有一个（IIb）仅在葡萄牙被发现。来自牛和野生反刍动物的分离株聚集在两个等位基因中。相比之下，人类分离株聚集在7个等位基因中，表现出广泛的等位基因多样性。
