Nikolettos Nikos, Asimakopoulos Byron, Tentes Loannis, Schöpper Beatte, al-Hasani Safaa
Lab. of Reproductive Physiology-IVF, Faculty of Medicine, Demokritus Univ. of Thrace, Ktirio Proklinikon, Dragana, 68100 Alexandroupolis, Greece.
In Vivo. 2003 Mar-Apr;17(2):201-4.
The objective of this study was to detect the level of matrix metalloproteinases 2 and 9 in the follicular fluid of patients undergoing controlled ovarian stimulation and to correlate these levels with hormonal and clinical parameters. Follicular fluids were collected from thirty patients participating in an intracytoplasmic sperm injection/embryo transfer (ICSI/ET) program in "Otmar Bauer" Assisted Reproduction Center, Alexandroupolis, Greece. Follicular fluid was collected at oocyte retrieval and MMP-2 and MMP-9 were identified by gelatin zymography. Gelatin zymography of the follicular fluids revealed bands consistent with proMMP-2 and proMMP-9. ProMMP-2 was the predominant band in the majority of samples. The levels of MMPs varied considerably among the samples. A significant positive correlation between MMP-2 and the peak estradiol levels was found.
本研究的目的是检测接受控制性卵巢刺激患者卵泡液中基质金属蛋白酶2和9的水平，并将这些水平与激素和临床参数相关联。卵泡液取自参与希腊亚历山德鲁波利斯“奥特马尔·鲍尔”辅助生殖中心卵胞浆内单精子注射/胚胎移植（ICSI/ET）项目的30名患者。在取卵时收集卵泡液，并用明胶酶谱法鉴定MMP-2和MMP-9。卵泡液的明胶酶谱显示出与proMMP-2和proMMP-9一致的条带。在大多数样本中，proMMP-2是主要条带。样本间MMPs水平差异很大。发现MMP-2与雌二醇峰值水平之间存在显著正相关。
