Asimakopoulos Byron, Abu-Hassan Deena, Metzen Eric, Al-Hasani Safaa, Diedrich Klaus, Nikolettos Nikos
Laboratory of Physiology, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.
Fertil Steril. 2008 Jul;90(1):60-4. doi: 10.1016/j.fertnstert.2007.05.054. Epub 2007 Nov 5.
To investigate the association between the levels of two steroid hormones and eight cytokines in fluids from individual follicles and the fertilization outcome of the oocytes derived from the same follicles.
Prospective study.
University hospital.
PATIENT(S): Forty-three women participating in intracytoplasmic sperm injection (ICSI)/ET cycles.
INTERVENTION(S): The ovarian stimulation followed the multidose GnRH antagonist protocol. ICSI was performed in mature oocytes. The concentrations of estradiol, progesterone, tumor necrosis factor-alpha, interleukin (IL) -1beta, IL-6, vascular endothelial growth factor, leptin, basic fibroblast growth factor, epidermal growth factor, and insulin-like growth factor-I were measured by immunoassay methods in the follicles from which the mature oocytes were derived.
MAIN OUTCOME MEASURE(S): The concentrations of the above hormones and cytokines in individual follicles and the fertilization outcome of the oocytes derived from the same follicles.
RESULT(S): The intrafollicular concentrations of the above factors were not significantly associated with the fertilization outcome. These factors were not correlated with embryo quality, with the exception of leptin, which was weakly associated with embryo score (R = 0.276).
CONCLUSION(S): The intrafollicular concentrations of the above factors cannot predict the fertilization outcome after ICSI.
研究单个卵泡液中两种甾体激素和八种细胞因子水平与源自同一卵泡的卵母细胞受精结局之间的关联。
前瞻性研究。
大学医院。
43名参与卵胞浆内单精子注射（ICSI）/胚胎移植（ET）周期的女性。
采用多剂量GnRH拮抗剂方案进行卵巢刺激。对成熟卵母细胞进行ICSI。采用免疫测定法测量源自成熟卵母细胞的卵泡中雌二醇、孕酮、肿瘤坏死因子-α、白细胞介素（IL）-1β、IL-6、血管内皮生长因子、瘦素、碱性成纤维细胞生长因子、表皮生长因子和胰岛素样生长因子-I的浓度。
单个卵泡中上述激素和细胞因子的浓度以及源自同一卵泡的卵母细胞的受精结局。
上述因素的卵泡内浓度与受精结局无显著关联。这些因素与胚胎质量无关，但瘦素除外，瘦素与胚胎评分呈弱相关（R = 0.276）。
上述因素的卵泡内浓度无法预测ICSI后的受精结局。
