Yang Jinbo, Xiao Chengfeng, Yang Xiaobo, Xu Qian
Institute of Occupational Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Wei Sheng Yan Jiu. 2003 Mar;32(2):92-4.
In order to investigate the relationship between proliferative activities and heat stress protein 70(HSP70) expression of porcine aortic endothelial cells(ECs) treated by benzo(a) pryene in vitro, porcine aortic endothelial cells were cultured in vitro, treated by different concentration of BaP(0, 0.5, 1, 10, 20 mumol/L) for 24 hours. Then, the proliferative activities and HSP70 expression of endothelial cells were determined by MTT and Western-blot respectively. The results showed that along with the rise of concentration of BaP, proliferative activities of porcine aortic endothelial cells decreased. There are not significantly difference of expression of HSP70 between and control and low-exposed cultures(0.5 mumol/L), but decreased at intermedieate and high concentration(1, 10, 20 mumol/L). It was suggested that BaP restrained proliferative activities of porcine aortic endothelial cells, which may be related to the inhibition of HSP70 expression by BaP.
为了研究体外苯并(a)芘处理的猪主动脉内皮细胞（ECs）增殖活性与热应激蛋白70（HSP70）表达之间的关系，体外培养猪主动脉内皮细胞，用不同浓度的BaP（0、0.5、1、10、20μmol/L）处理24小时。然后，分别通过MTT和Western-blot法测定内皮细胞的增殖活性和HSP70表达。结果表明，随着BaP浓度的升高，猪主动脉内皮细胞的增殖活性降低。对照组和低暴露组（0.5μmol/L）之间HSP70表达无显著差异，但在中、高浓度（1、10、20μmol/L）时降低。提示BaP抑制猪主动脉内皮细胞的增殖活性，这可能与BaP对HSP70表达的抑制有关。
