Bernardini Chiara, Zannoni Augusta, Turba Maria Elena, Bacci Maria Laura, Forni Monica, Mesirca Pietro, Remondini Daniel, Castellani Gastone, Bersani Ferdinando
Department of Veterinary Morphophysiology and Animal Production DIMORFIPA, Bologna University, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy.
Bioelectromagnetics. 2007 Apr;28(3):231-7. doi: 10.1002/bem.20299.
The aim of the present study was to investigate the influence of 50 Hz sinusoidal magnetic field on Hsp27, Hsp70, and Hsp90 expression in a model of primary culture of porcine aortic endothelial cells (PAEC). We took into consideration the Hsp profile in terms of mRNA expression, protein expression and protein localization inside the cells. The choice of the cell system was motivated by the involvement of the endothelial cells in the onset of many diseases; moreover, only few reports describe the effects of extremely low frequency magnetic fields (ELF-MFs) on such cells. ELF-MF exposure induced an increase in the mRNA levels of the three proteins, which was statistically significant for Hsp70. On the contrary, we did not observe any influence on Hsp27, Hsp70, and Hsp90 protein levels. Analysis in situ by immunofluorescence revealed that ELF-MF exposure affected the cellular distribution of Hsp27; in particular a partial relocalization in the nucleus was observed.
本研究的目的是在猪主动脉内皮细胞（PAEC）原代培养模型中，研究50 Hz正弦磁场对热休克蛋白27（Hsp27）、热休克蛋白70（Hsp70）和热休克蛋白90（Hsp90）表达的影响。我们从mRNA表达、蛋白质表达以及细胞内蛋白质定位方面考虑热休克蛋白谱。选择该细胞系统的原因是内皮细胞参与多种疾病的发生；此外，仅有少数报告描述了极低频磁场（ELF-MFs）对此类细胞的影响。暴露于ELF-MF会导致这三种蛋白质的mRNA水平升高，其中Hsp70的升高具有统计学意义。相反，我们未观察到对Hsp27、Hsp70和Hsp90蛋白质水平有任何影响。免疫荧光原位分析显示，暴露于ELF-MF会影响Hsp27的细胞分布；特别是观察到其在细胞核中有部分重新定位。
