Song Xiurong, Yang Gengliang, Zhao Jingxiang, Chen Yi, Sun Hanwen, Zhang Hongyi
Department of Chemistry, Hebei University, Baoding 071002, People's Republic of China.
Phytochem Anal. 2003 May-Jun;14(3):132-6. doi: 10.1002/pca.692.
A micellar electrokinetic capillary chromatographic (MEKC) method has been established for the identification and determination of diterpenoid triepoxides in the Chinese herb Tripterygium wilfordii Hook F. and its preparations. Studies of the influence of boric acid and borax buffer concentration and pH, and of sodium dodecylsulphate (SDS) concentration have been carried out, and the optimum separation for the triepoxides was achieved using 20 mM boric acid and 10 mM borax with 20 mM SDS as the running buffer. MEKC was found to exhibit good accuracy, precision and repeatability. The sensitivity of the assay was sufficient to monitor the three active components in T. wilfordii and its preparations.
已建立一种胶束电动毛细管色谱（MEKC）方法，用于鉴定和测定中药雷公藤及其制剂中的二萜三环氧物。研究了硼酸和硼砂缓冲液浓度、pH值以及十二烷基硫酸钠（SDS）浓度的影响，以20 mM硼酸、10 mM硼砂和20 mM SDS作为运行缓冲液时，实现了三环氧物的最佳分离。结果表明，MEKC具有良好的准确性、精密度和重复性。该测定方法的灵敏度足以监测雷公藤及其制剂中的三种活性成分。
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