Wu Yi-Wei, Jiang Yin-Yan, Xiao Tang-Xin, Zhang Hai-Li
Department of Chemistry and Environmental Engineering, Hubei Key Laboratory of Bioanalytical Technology, Hubei Normal University, Huangshi, PR China.
J Sep Sci. 2008 Mar;31(5):865-71. doi: 10.1002/jssc.200700463.
A method has been developed for the determination of triptonide in the traditional Chinese herb Tripterygium wilfordii Hook F. by micellar electrokinetic capillary chromatography combined with cloud point extraction. The analyte was extracted at pH 3.0 by micelles of the nonionic surfactant polyoxyethylene 7,5-octylphenyl ether (Triton X-114). A 250-muL aliquot from the extracted surfactant-rich phase was diluted to 400 muL with ethanol to reduce its viscosity before separation by MEKC. Under optimum conditions, an enrichment factor of 25 is obtained and the determination limit of triptonide is found to be 3.15 x 10(-7) mol/L. The proposed method has been successfully applied to the determination of triptonide in T. wilfordii tablet and spiked urine matrix, demonstrating the feasibility and reliability of the proposed method.
已开发出一种采用胶束电动毛细管色谱结合浊点萃取法测定传统中药雷公藤（Tripterygium wilfordii Hook F.）中雷公藤内酯醇的方法。在pH 3.0条件下，用非离子表面活性剂聚氧乙烯7,5 - 辛基苯基醚（Triton X - 114）的胶束萃取分析物。从富含表面活性剂的萃取相中取出250 μL等分试样，用乙醇稀释至400 μL以降低其粘度，然后通过胶束电动毛细管色谱进行分离。在最佳条件下，获得了25的富集因子，雷公藤内酯醇的测定限为3.15×10⁻⁷ mol/L。该方法已成功应用于雷公藤片剂和加标尿液基质中雷公藤内酯醇的测定，证明了该方法的可行性和可靠性。
