Li Baozhi, Bai Jie, Yang Gengliang, Li Zhiwei, Wang Lijuan, Chen Yi
College of Pharmacy, Hebei University, Baoding 071002, China.
J Chromatogr A. 2005 Dec 2;1097(1-2):199-202. doi: 10.1016/j.chroma.2005.06.043. Epub 2005 Nov 8.
A novel concocting method to convert Triptolide (T) into Tripchlorolide (T(4)) in the traditional Chinese herb Tripterygium wilfordii Hook F. and a micellar electrokinetic capillary chromatographic (MEKC) approach by which the conversion of Triptolide (T) and Tripchlorolide (T(4)) was identified and determined had been established. Investigations of the influence of different pH values of boric acid and borax buffer and of sodium dodecyl sulfate (SDS) and organic additive concentrations had been carried out, and the optimum separation for T and T(4) was achieved using boric acid and borax of pH 7.0 with 30 mM SDS and 20% (volume ratio) methanol as the running buffer. It was found that MEKC exhibited good accuracy, precision and repeatability and the content of T(4) was greatly increased in the herb that was treated by the new concocting method.
一种将传统中药雷公藤中的雷公藤甲素（T）转化为雷公藤氯内酯醇（T(4)）的新型炮制方法以及一种用于鉴定和测定雷公藤甲素（T）和雷公藤氯内酯醇（T(4)）转化情况的胶束电动毛细管色谱（MEKC）方法已经建立。研究了硼酸和硼砂缓冲液不同pH值以及十二烷基硫酸钠（SDS）和有机添加剂浓度的影响，使用pH 7.0的硼酸和硼砂、30 mM SDS和20%（体积比）甲醇作为运行缓冲液实现了T和T(4)的最佳分离。结果发现，MEKC具有良好的准确性、精密度和重复性，并且采用新炮制方法处理的草药中T(4)的含量大幅增加。
