Caux C, Dezutter-Dambuyant C, Schmitt D, Banchereau J
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
Nature. 1992 Nov 19;360(6401):258-61. doi: 10.1038/360258a0.
Dendritic cells comprise a system of highly efficient antigen-presenting cells which initiate immune responses such as the sensitization of T cells restricted by major histocompatibility complex molecules, the rejection of organ transplants and the formation of T-cell-dependent antibodies. Dendritic cells are found in many non-lymphoid tissues, such as skin (Langerhans cells) and mucosa, and they migrate after antigen capture through the afferent lymph or the bloodstream to lymphoid organs, where they efficiently present antigen to T cells. Dendritic cells are difficult to isolate and, although they originate from bone marrow their site of maturation and the conditions that direct their growth and differentiation are still poorly characterized. Granulocyte macrophage-colony stimulating factor (GM-CSF) favours the outgrowth of dendritic cells from mouse peripheral blood. Here we extend this finding to man and demonstrate that cooperation between GM-CSF and tumour necrosis factor-alpha (TNF-alpha) is crucial for the generation of human dendritic/Langerhans cells from CD34+ haematopoietic progenitors. The availability of large numbers of these cells should now facilitate the understanding of their role in immunological regulation and disorder.
树突状细胞构成了一个高效的抗原呈递细胞系统，该系统可启动免疫反应，如由主要组织相容性复合体分子限制的T细胞致敏、器官移植排斥反应以及T细胞依赖性抗体的形成。树突状细胞存在于许多非淋巴组织中，如皮肤（朗格汉斯细胞）和黏膜，它们在捕获抗原后通过输入淋巴管或血液循环迁移至淋巴器官，在那里它们有效地将抗原呈递给T细胞。树突状细胞难以分离，尽管它们起源于骨髓，但其成熟部位以及指导其生长和分化的条件仍未得到充分描述。粒细胞巨噬细胞集落刺激因子（GM-CSF）有利于小鼠外周血中树突状细胞的生长。在此，我们将这一发现扩展至人类，并证明GM-CSF与肿瘤坏死因子-α（TNF-α）之间的协同作用对于从CD34+造血祖细胞生成人类树突状/朗格汉斯细胞至关重要。现在，大量此类细胞的可得性应有助于理解它们在免疫调节和疾病中的作用。
