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用于检测HIV抗体的快速全血手指采血检测:泰国北部女性中的性能与可接受性

Rapid whole-blood finger-stick test for HIV antibody: performance and acceptability among women in northern Thailand.

作者信息

Liu Alice, Kilmarx Peter H, Supawitkul Somsak, Chaowanachan Thanyanan, Yanpaisarn Somboonsak, Chaikummao Supaporn, Limpakarnjanarat Khanchit

机构信息

Thailand Ministry of Public Health-US Centers for Disease Control and Prevention Collaboration, DMS Building 6, Ministry of Public Health, Nonthaburi 11000, Thailand.

出版信息

J Acquir Immune Defic Syndr. 2003 Jun 1;33(2):194-8. doi: 10.1097/00126334-200306010-00013.

Abstract

Although use of rapid HIV antibody tests of finger-stick blood specimens could expand voluntary counseling and testing in areas where fear of venipuncture and delays in learning test results are barriers, there is little information on performance and acceptability of these tests in Asia. We used the Hema. Strip HIV-1/2 test (Saliva Diagnostic Systems, Vancouver, WA) in a prospective cohort study of HIV seroincidence among women in northern Thailand from 1998 to 1999. Nurses obtained whole-blood specimens by finger-stick testing and provided test results and counseling at each visit. Acceptability of the rapid test was assessed at the first 6-month follow-up visit. HIV-1 seroprevalence among the 804 women screened at enrollment was 3.1%. Positive rapid test results from 25 women were confirmed by enzyme immunoassay and Western blot analysis using serum obtained by venipuncture. Of the 741 women who returned for follow-up, 56% preferred specimen collection by finger-stick testing to venipuncture, 80% preferred immediate rather than delayed test results, 79% preferred the rapid test method to typical testing methods, and 97% were satisfied with the test method used. Results from this study demonstrate the utility and acceptability of the rapid finger-stick test for HIV antibody among women in northern Thailand.

摘要

虽然使用手指采血标本进行快速HIV抗体检测可以在那些因害怕静脉穿刺和等待检测结果时间过长而成为障碍的地区扩大自愿咨询检测服务,但在亚洲,关于这些检测的性能和可接受性的信息却很少。在1998年至1999年对泰国北部妇女进行的一项HIV血清发病率前瞻性队列研究中,我们使用了Hema. Strip HIV-1/2检测(唾液诊断系统公司,华盛顿州温哥华)。护士通过手指采血获取全血标本,并在每次就诊时提供检测结果和咨询服务。在首次6个月随访就诊时评估快速检测的可接受性。在入组时接受筛查的804名妇女中,HIV-1血清阳性率为3.1%。25名妇女的快速检测阳性结果通过酶免疫测定和使用静脉穿刺获得的血清进行的免疫印迹分析得到确认。在741名返回进行随访的妇女中,56%的人更喜欢通过手指采血而不是静脉穿刺采集标本,80%的人更喜欢即时而非延迟的检测结果,79%的人更喜欢快速检测方法而非传统检测方法,97%的人对所使用的检测方法感到满意。这项研究的结果证明了快速手指采血检测在泰国北部妇女中用于HIV抗体检测的实用性和可接受性。

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