Fu Lijuan, Abu-Khalil Amir, Morrison Richard S, Geschwind Daniel H, Kornblum Harley I
Department of Neurology, University of California, Los Angeles School of Medicine, Los Angeles, California 90095, USA.
J Comp Neurol. 2003 Jul 21;462(2):265-73. doi: 10.1002/cne.10727.
Factors that interact with the epidermal growth factor and fibroblast growth factor receptors have numerous effects in the central nervous system (CNS), inducing the proliferation of CNS stem cells and astrocytes and the survival and differentiation of neurons. Both receptors are expressed in the embryonic rodent brain in proliferative and nonproliferative regions, suggesting roles in numerous developmental processes. However, the roles of these factors in human brain development are not known. In the current study, we examined the expression of human epidermal growth factor receptor (HEGFR) and human fibroblast growth factor receptor 1 (HFGFR1) mRNAs in the human fetal brain. The expression of both receptors is strikingly conserved relative to previously reported patterns in the rodent. In the germinal zones, the sites of cellular proliferation, HFGFR1 was expressed primarily in the ventricular zone, whereas HEGFR was expressed in the subventricular zone, suggesting different roles in CNS progenitor proliferation. Differential expression was also observed in other brain areas examined, including the hippocampus and the cerebellum. The current study suggests that HEGFR and HFGFR1 are likely to play different roles during human brain development, but that these roles will be similar to those observed in the rodent brain.
与表皮生长因子和成纤维细胞生长因子受体相互作用的因子在中枢神经系统（CNS）中有多种作用，可诱导中枢神经系统干细胞和星形胶质细胞的增殖以及神经元的存活和分化。这两种受体在胚胎期啮齿动物脑的增殖区和非增殖区均有表达，提示它们在众多发育过程中发挥作用。然而，这些因子在人类脑发育中的作用尚不清楚。在本研究中，我们检测了人胎儿脑中人类表皮生长因子受体（HEGFR）和人类成纤维细胞生长因子受体1（HFGFR1）mRNA的表达。相对于先前报道的啮齿动物中的模式，这两种受体的表达具有显著的保守性。在生发区，即细胞增殖的部位，HFGFR1主要在脑室区表达，而HEGFR在室下区表达，提示它们在中枢神经系统祖细胞增殖中发挥不同作用。在包括海马体和小脑在内的其他检测脑区也观察到了差异表达。本研究表明，HEGFR和HFGFR1在人类脑发育过程中可能发挥不同作用，但这些作用可能与在啮齿动物脑中观察到的作用相似。
