Jaffré Britta, Watrin Astrid, Loeuille Damien, Gillet Pierre, Netter Patrick, Laugier Pascal, Saïed Amena
LIP, Unité Mixte de Recherches (UMR) 7623 CNRS-Université Paris VI, Paris, France.
Arthritis Rheum. 2003 Jun;48(6):1594-601. doi: 10.1002/art.11023.
To evaluate the ability of 55-MHz quantitative ultrasound (US) to detect the in vivo effects of experimental arthritis, as well as those of two antiinflammatory drugs, naproxen (NPX) and dexamethasone (DEX), on cartilage and subchondral bone.
Arthritis was induced in both knees of 108 rats by intraarticular injection of zymosan (ZYM). Two groups of arthritic rats (n = 36 per group) were treated daily with either NPX (10 mg/kg/day) or DEX (0.1 mg/kg/day). Using a 3-dimensional US microscope, patellae were explored in vitro on days 5, 14, and 21 after injections. US assessment included the analysis of quantitative indices of local modifications involving cartilage and bone: integrated reflection coefficient (IRC) from the cartilage surface and apparent integrated backscatter from the cartilage internal structure (cartilage matrix) (AIB(cartilage)) and the cartilage-bone interface (AIB(bone)).
ZYM induced articular surface fibrillation that resulted in a decrease in IRC at all times (P < 0.02) and in an increase in AIB(bone) on days 5 and 14 (P < 0.005). Fibrillation was not changed by NPX administration, while it disappeared following DEX treatment. Cartilage-bone interface alterations were prevented by DEX and partially compensated for by NPX. Cartilage matrix echogenicity decreased with time in all groups due to maturation (P < 0.05), except in DEX-treated rats.
Quantitative 55 MHz US allowed detection of early cartilage and bone lesions due to experimental arthritis, and also allowed detection of the effects of antiinflammatory drugs. NPX seemed to have an effect on subchondral bone lesions, but not on cartilage. DEX appeared to repair articular surface and bone, but prevented animal growth and cartilage maturation.
评估55兆赫定量超声（US）检测实验性关节炎的体内效应以及两种抗炎药物萘普生（NPX）和地塞米松（DEX）对软骨和软骨下骨的体内效应的能力。
通过关节内注射酵母聚糖（ZYM）在108只大鼠的双膝关节诱发关节炎。两组关节炎大鼠（每组n = 36）每天分别用NPX（10毫克/千克/天）或DEX（0.1毫克/千克/天）治疗。在注射后第5、14和21天，使用三维超声显微镜对髌骨进行体外探测。超声评估包括分析涉及软骨和骨的局部改变的定量指标：软骨表面的积分反射系数（IRC）以及软骨内部结构（软骨基质）（AIB（软骨））和软骨-骨界面（AIB（骨））的表观积分背向散射。
ZYM诱发关节表面纤维性颤动，导致IRC在所有时间均降低（P < 0.02），并且在第5天和第14天AIB（骨）增加（P < 0.005）。给予NPX后纤维性颤动未改变，而在DEX治疗后消失。DEX可预防软骨-骨界面改变，NPX可部分代偿。由于成熟，所有组中软骨基质回声均随时间降低（P < 0.05），DEX治疗的大鼠除外。
55兆赫定量超声能够检测实验性关节炎引起的早期软骨和骨病变，也能检测抗炎药物的效应。NPX似乎对软骨下骨病变有影响，但对软骨无影响。DEX似乎可修复关节表面和骨，但会抑制动物生长和软骨成熟。
