Tucker A W, McNeilly F, Meehan B, Galbraith D, McArdle P D, Allan G, Patience C
Imutran Ltd. (A Novartis Pharma AG Co.), Cambridge, UK.
Xenotransplantation. 2003 Jul;10(4):343-8. doi: 10.1034/j.1399-3089.2003.02048.x.
The use of porcine tissues is being developed as a means to alleviate the shortage of allogeneic tissues and organs available for transplantation. To reduce the possibility of a microorganism of pigs being inadvertently transferred to the recipient of the xenograft, recommendations have been published on the microbiological specifications for organ source pigs. The porcine circoviruses (PCV1 and PCV2) and porcine lymphotropic herpesviruses (PLHV1 and PLHV2) are two infectious agents of pigs which are considered to be of significance for the microbiological safety of xenotransplantation. To ensure the exclusion of these microorganisms from animals destined for use under clinical conditions, reliable breeding methodologies are required. We investigated the efficiency of established derivation procedures for the removal of PCV and PLHV. In comparison with conventionally reared pigs, caesarian and barrier derived animals showed a markedly reduced prevalence of PCVs and PLHVs. Our results indicate that the derivation of animals free of both of these microorganisms is achievable and will enhance the microbiological safety of xenotransplantation.
猪组织的应用正在作为一种手段加以发展，以缓解可用于移植的同种异体组织和器官的短缺。为降低猪的微生物意外转移到异种移植受者身上的可能性，已发布了关于器官源猪微生物学规范的建议。猪圆环病毒（PCV1和PCV2）和猪嗜淋巴细胞疱疹病毒（PLHV1和PLHV2）是猪的两种感染因子，它们被认为对异种移植的微生物安全具有重要意义。为确保在临床条件下使用的动物排除这些微生物，需要可靠的繁育方法。我们研究了既定的去除PCV和PLHV的培育程序的效率。与传统饲养的猪相比，剖腹产和屏障培育的动物中PCV和PLHV的流行率显著降低。我们的结果表明，培育出不含这两种微生物的动物是可行的，这将提高异种移植的微生物安全性。
