严重急性呼吸综合征（SARS）相关病毒的形态学与形态发生

Morphology and morphogenesis of severe acute respiratory syndrome (SARS)-associated virus.

作者信息

Zhang Qin-Fen, Cui Jin-Ming, Huang Xiao-Jun, Lin Wei, Tan Dong-Yan, Xu Jie-Wei, Yang Yi-Feng, Zhang Jing-Qiang, Zhang Xin, Li Hui, Zheng Huan-Ying, Chen Qiu-Xia, Yan Xin-Ge, Zheng Kui, Wan Zhuo-Yue, Huang Jie-Cheng

机构信息

State Key Lab for Biocontrol, Zhongshan University, Guangzhou 510275, China.

出版信息

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2003 Jun;35(6):587-91.

PMID:12796822

Abstract

After infecting the Vero E6 cells by nasal/throat swabs collected from SARS patients, we studied the SARS-associated virus by electron microscopy and molecular biological technique. The results show that the diameter of newly isolated virus is about 50 nm without envelope or 100 nm with envelope. The virus was proved to be a new coronavirus by RT-PCR and it responded positively to convalescent-phase serum specimen from SARS patients, which is the evidence that this new virus is etiologically linked to the outbreak of SARS. The morphogenesis and distribution of the virus are also discussed in this article.

摘要

用从非典患者采集的鼻/咽拭子感染Vero E6细胞后，我们通过电子显微镜和分子生物学技术研究了与非典相关的病毒。结果显示，新分离出的病毒直径约为50纳米（无包膜）或100纳米（有包膜）。通过逆转录聚合酶链反应（RT-PCR）证明该病毒为一种新型冠状病毒，并且它对非典患者的恢复期血清标本呈阳性反应，这是这种新病毒在病因上与非典爆发有关的证据。本文还讨论了该病毒的形态发生和分布情况。