Angelov Borislav, Angelova Angelina, Ollivon Michel, Bourgaux Claudie, Campitelli Andrew
Institute of Biophysics, Bulgarian Academy of Sciences, Acad. G.Bonchev Str. Bl.21, BG-1113 Sofia, Bulgaria.
J Am Chem Soc. 2003 Jun 18;125(24):7188-9. doi: 10.1021/ja034578v.
This paper describes a diamond cubic phase with large water channels and determines the temperature dependence of the bilayer thickness in the cubic monoolein/octylglucoside/water system based on time-resolved synchrotron X-ray diffraction data. The X-ray diffraction study established a diamond-type lipid cubic phase with large water channels (Dlarge), which has not been previously reported. It is a distinct phase, different from the diamond cubic phase with normal water channels (Dnormal). The larger channels might allow an enhanced entrapment efficiency of biomolecules in lipid cubic phases. The X-ray diffraction patterns recorded during a thermal scan showed a cubic-cubic structural transition from Dlarge to Dnormal. The obtained cubic phases displayed much larger lattice spacings as compared to those of pure monoolein at full hydration.
本文描述了一种具有大的水通道的金刚石立方相，并基于时间分辨同步辐射X射线衍射数据确定了立方单油酸甘油酯/辛基葡糖苷/水体系中双层厚度的温度依赖性。X射线衍射研究确定了一种具有大的水通道的金刚石型脂质立方相（Dlarge），这是以前未曾报道过的。它是一个独特的相，不同于具有正常水通道的金刚石立方相（Dnormal）。较大的通道可能会提高生物分子在脂质立方相中的包封效率。在热扫描过程中记录的X射线衍射图谱显示了从Dlarge到Dnormal的立方-立方结构转变。与完全水合的纯单油酸甘油酯相比，所获得的立方相显示出大得多的晶格间距。
