Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
Departments of Materials and Bioengineering and Institute of Biomedical Engineering, Imperial College London, London, UK.
Angew Chem Int Ed Engl. 2019 Mar 4;58(10):2958-2978. doi: 10.1002/anie.201804067. Epub 2018 Sep 26.
Cubosomes are highly stable nanoparticles formed from the lipid cubic phase and stabilized by a polymer based outer corona. Bicontinuous lipid cubic phases consist of a single lipid bilayer that forms a continuous periodic membrane lattice structure with pores formed by two interwoven water channels. Cubosome composition can be tuned to engineer pore sizes or include bioactive lipids, the polymer outer corona can be used for targeting and they are highly stable under physiological conditions. Compared to liposomes, the structure provides a significantly higher membrane surface area for loading of membrane proteins and small drug molecules. Owing to recent advances, they can be engineered in vitro in both bulk and nanoparticle formats with applications including drug delivery, membrane bioreactors, artificial cells, and biosensors. This review outlines recent advances in cubosome technology enabling their application and provides guidelines for the rational design of new systems for biomedical applications.
立方体贴是由脂质立方相形成的高度稳定的纳米颗粒，并通过聚合物外层稳定。双连续脂质立方相由单个脂质双层组成，形成具有由两个交织的水通道形成的孔的连续周期性膜晶格结构。立方体贴的组成可以进行调整以控制孔径大小或包含生物活性脂质，聚合物外层可以用于靶向，并且在生理条件下高度稳定。与脂质体相比，该结构为膜蛋白和小分子药物的装载提供了显著更高的膜表面积。由于最近的进展，它们可以在块状和纳米颗粒形式的体外进行工程设计，应用包括药物输送、膜生物反应器、人工细胞和生物传感器。本文综述了立方体贴技术的最新进展，使其能够应用，并为生物医学应用的新系统的合理设计提供了指导。
