Frydas S, Papazahariadou M, Papaioannou N, Hatzistilianou M, Trakatellis M, Merlitti D, Di Gioacchino M, Grilli A, DeLutiis M A, Riccioni G, Conti P, Vlemmas I
Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki 540 06, Greece.
Int J Immunopathol Pharmacol. 2003 May-Aug;16(2):99-104. doi: 10.1177/039463200301600202.
The plant amino acid L-mimosine has recently been suggested to inhibit cells at a regulatory step in late G1 phase before establishment of active DNA replication forks. In addition, L-mimosine is an extremely effective inhibitor of DNA replication in chromosomes of mammalian nuclei. In this work, the effect of L-mimosine on chronic inflammation induced by dorsal injections of 0.2 ml of a 1:40 saturated crystal solution of potassium permanganate in mice, was studied. Seven days afterwards, all mice developed a subcutaneous granulomatous tissue indicative of chronic inflammatory response at the site of infection. The intraperitoneal administration of L-mimosine (200 microg/dose) to the potassium permanganate treated mice for 5 consecutive days (the first at the same time of inoculation of the KMnO4), produced a significant decrease in size and weight of the granuloma when compared to mice not treated with L-mimosine (controls). In addition, in all mice treated with L-mimosine, there was a strong inhibition of tumor necrosis factor alpha that was revealed in the serum (P<0.05) and in the minced granulomas. Interleukin-6 was not detected in the serum of treated and untreated mice. These findings show for the first time, that L-mimosine may have an anti-inflammatory effect on chronic inflammation and an inhibitory effect on tumor necrosis factor alpha and interleukin-6 generation in supernatant fluids of minced granulomas.
最近有人提出，植物氨基酸L-含羞草碱在活性DNA复制叉形成之前的G1期晚期的一个调控步骤中抑制细胞。此外，L-含羞草碱是哺乳动物细胞核染色体中DNA复制的一种极其有效的抑制剂。在这项研究中，研究了L-含羞草碱对小鼠背部注射0.2 ml 1:40高锰酸钾饱和晶体溶液诱导的慢性炎症的影响。七天后，所有小鼠在感染部位都出现了皮下肉芽肿组织，表明存在慢性炎症反应。向经高锰酸钾处理的小鼠连续5天腹腔注射L-含羞草碱（200微克/剂量）（第一次与接种KMnO4同时进行），与未用L-含羞草碱处理的小鼠（对照组）相比，肉芽肿的大小和重量显著减小。此外，在所有用L-含羞草碱处理的小鼠中，血清（P<0.05）和切碎的肉芽肿中肿瘤坏死因子α受到强烈抑制。在处理和未处理小鼠的血清中均未检测到白细胞介素-6。这些发现首次表明，L-含羞草碱可能对慢性炎症具有抗炎作用，并对切碎的肉芽肿上清液中的肿瘤坏死因子α和白细胞介素-6生成具有抑制作用。
