Conti P, Feliciani C, Barbacane R C, Frydas S, Placido F C, Cataldo I, Reale M
Immunology Division, Department of Oncology and Neuroscience, University of Chieti Medical School, 66013 Chieti, Italy.
Calcif Tissue Int. 1999 Jan;64(1):57-62. doi: 10.1007/s002239900579.
Monocyte chemotactic protein-1 (MCP-1) and related molecules constitute the C-C class of the beta chemokine supergene family with inflammatory properties. However, the exact role, function, and implication in inflammatory diseases remain to be determined. Here we report that subcutaneous injections (0.2 ml) of a saturated water solution (1:40) of potassium permanganate crystals induces the generation of granuloma tissue at the site of injection in the rat, and reaches its peak of formation after 1 week. The size and weight of the granulomas were increased by i.p. lipopolysaccharide (LPS) (6 microgram/200 microliter) and inhibited by intraperitoneal (i.p.) dexamethasone (Dxs) 300 microgram/200 microliter) treatments in rats, injected 18 hours before sacrifice. Moreover, steady-state levels of MCP-1 mRNA in the granuloma tissue (control), were strongly generated. Rats treated i.p. with LPS produced an increase of MCP-1 mRNA in the granuloma tissue compared with controls (i.p. PBS-treated) whereas in animals treated with Dxs, there was a decrease in (P < 0.05) in formation of mRNA protein. When the granuloma tissues were homogenized the generation of MCP-1 was found in the supernatants. The level of MCP-1 was higher (P < 0.05) in the LPS-treated animals and lower (P < 0.05) in the Dxs group compared with the controls (treated with PBS). Similar results were obtained in the serum and in minced granuloma tissue where samples were further incubated in vitro with LPS (100 ng/ml) overnight. A Strong increase (P < 0.01) in MCP-1 in all samples was detected, but not in the minced granuloma tissue from Dxs-treated animals. Our data demonstrate that calcified tissue from chronic inflammation induced by KMnO4 generates MCP-1 gene expression and translation, an effect increased by LPS and decreased by Dxs.
单核细胞趋化蛋白-1（MCP-1）及相关分子构成具有炎症特性的β趋化因子超基因家族的C-C类。然而，其在炎症性疾病中的确切作用、功能及影响仍有待确定。在此我们报告，皮下注射（0.2毫升）高锰酸钾晶体饱和水溶液（1:40）可诱导大鼠注射部位形成肉芽肿组织，并在1周后达到形成高峰。肉芽肿的大小和重量在腹腔注射脂多糖（LPS）（6微克/200微升）后增加，而在处死前18小时腹腔注射地塞米松（Dxs）300微克/200微升后受到抑制。此外，肉芽肿组织（对照组）中MCP-1 mRNA的稳态水平强烈产生。腹腔注射LPS的大鼠与对照组（腹腔注射PBS）相比，肉芽肿组织中MCP-1 mRNA增加，而在用Dxs治疗的动物中，mRNA蛋白形成减少（P<0.05）。当肉芽肿组织匀浆时，发现上清液中有MCP-1产生。与对照组（用PBS处理）相比，LPS处理的动物中MCP-1水平较高（P<0.05），Dxs组中较低（P<0.05）。在血清和切碎的肉芽肿组织中也获得了类似结果，其中样品在体外与LPS（100纳克/毫升）进一步孵育过夜。所有样品中均检测到MCP-1强烈增加（P<0.01），但在Dxs处理动物的切碎肉芽肿组织中未检测到。我们的数据表明，由KMnO4诱导的慢性炎症钙化组织产生MCP-1基因表达和翻译，LPS可增强该效应，而Dxs可减弱该效应。
