一种使用伞形酮酰氧基甲基醚的用于脂肪酶和酯酶的低背景高通量筛选（HTS）荧光测定法。
A low background high-throughput screening (HTS) fluorescence assay for lipases and esterases using acyloxymethylethers of umbelliferone.
作者信息
Leroy Emmanuel, Bensel Nicolas, Reymond Jean Louis
机构信息
Department of Chemistry & Biochemistry, University of Bern, Freiestrasse 3, 3012, Bern, Switzerland.
出版信息
Bioorg Med Chem Lett. 2003 Jul 7;13(13):2105-8. doi: 10.1016/s0960-894x(03)00377-9.
Abstract
Esters and acyloxymethyl ethers of umbelliferone were evaluated as fluorogenic substrates for lipases and esterases with respect to stability and resistance to non-specific hydrolysis. Isobutyryloxymethyl ether 1c and pivaloxymethyl ether 1d were found to be optimal substrates for enzyme assays, particularly with respect to HTS applications.
摘要
就稳定性和对非特异性水解的抗性而言，对伞形花内酯的酯类和酰氧基甲基醚作为脂肪酶和酯酶的荧光底物进行了评估。发现异丁酰氧基甲基醚1c和新戊酰氧基甲基醚ld是酶分析的最佳底物，特别是在高通量筛选应用方面。
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