Miyamoto Ryozo, Uzawa Narikazu, Nagaoka Shunya, Hirata Yasushi, Amagasa Teruo
Maxillofacial Surgery, Maxillofacial Reconstruction and Function, Division of Maxillofacial and Neck Reconstruction, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
Oral Oncol. 2003 Sep;39(6):610-8. doi: 10.1016/s1368-8375(03)00048-4.
Amplification and overexpression of cyclin D1 (CCND1) have been reported as independent prognostic indicators of several tumors. To investigate the association between CCND1 amplification and overexpression in oral squamous cell carcinomas (OSCCs), and to determine which is more reliable as a prognostic indicator, fluorescence in situ hybridization (FISH) on fine-needle aspiration (FNA) biopsies and immunohistochemistry were performed on 41 primary OSCCs (26 males, 15 females; mean age; 58.4 years, range 21-89 years). Thirteen patients were stage I, 13 were stage II, nine were stage III, and six were stage IV. CCND1 amplification and overexpression was detected in 13 (31.7%) and 27 (65.9%) of 41 cases. CCND1 was overexpressed in all cases showing CCND1 amplification. On the other hand, CCND1 overexpression was also detected in 14 of 28 cases (50.0%) lacked such amplification. Statistical analysis showed that the correlation between CCND1 overexpression and decreased survival just failed to reach statistical significance, and CCND1 amplification and nodal status were independent prognostic indicators. In conclusion, it will be necessary to investigate the other pathways that regulate CCND1 expression besides CCND1 amplification. From the present study, CCND1 amplification is a more reliable prognostic indicator than CCND1 overexpression in OSCCs.
细胞周期蛋白D1（CCND1）的扩增和过表达已被报道为多种肿瘤的独立预后指标。为了研究口腔鳞状细胞癌（OSCC）中CCND1扩增与过表达之间的关联，并确定哪一个作为预后指标更可靠，对41例原发性OSCC（26例男性，15例女性；平均年龄58.4岁，范围21 - 89岁）进行了细针穿刺（FNA）活检的荧光原位杂交（FISH）和免疫组织化学检测。13例患者为I期，13例为II期，9例为III期，6例为IV期。41例病例中，13例（31.7%）检测到CCND1扩增，27例（65.9%）检测到CCND1过表达。在所有显示CCND1扩增的病例中CCND1均过表达。另一方面，在28例缺乏这种扩增的病例中，有14例（50.0%）也检测到CCND1过表达。统计分析表明，CCND1过表达与生存率降低之间的相关性刚刚未达到统计学意义，并且CCND1扩增和淋巴结状态是独立的预后指标。总之，有必要研究除CCND1扩增之外调节CCND1表达的其他途径。从本研究来看，在OSCC中CCND1扩增是比CCND1过表达更可靠的预后指标。
