舌及颊部鳞状细胞癌中细胞周期蛋白D1的扩增

Cyclin D1 amplification in tongue and cheek squamous cell carcinoma.

作者信息

Mahdey Haydar M, Ramanathan Anand, Ismail Siti Mazlipah, Abraham Mannil Thomas, Jamaluddin Marhazlinda, Zain Rosnah Binti

机构信息

Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.

出版信息

Asian Pac J Cancer Prev. 2011;12(9):2199-204.

PMID:22296356

Abstract

INTRODUCTION

Several molecular markers have been studied for their usefulness as prognostic markers in oral squamous cell carcinoma (OSCC). One such molecular marker is cyclin D1 which is a proto-oncogene located on 11q13 in humans.

OBJECTIVE

To explore the feasibility of using cyclin D1 as a prognostic marker in tongue and cheek SCC by the fluorescent-in-situ hybridization (FISH) method.

METHODS

Fifty paraffin-embedded samples (25 each of cheek and tongue SCCs) were obtained from the archives of the Oral Pathology Diagnostic Laboratory. Sociodemographic data, histopathologic diagnoses, lymph node status and survival data were obtained from the Malaysian Oral Cancer Database and Tissue Bank System (MOCDTBS)coordinated by the Oral Cancer Research and Coordinating Centre (OCRCC), University of Malaya. The FISH technique was used to detect the amplification of cyclin D1 using the Vysis protocol. Statistical correlations of cyclin D1 with site and lymph node status were analyzed using the Fisher exact test. Kaplan-Meier and Log Rank (Mantel-Cox) test were used to analyze cyclin D1 amplification and median survival time.

RESULTS

Positive amplification of cyclin D1 was detected in 72% (36) of OSCCs. Detection of positive amplification for cyclin D1 was observed in 88% (22) and 56% (14) of the tongue and cheek tumors, respectively, where the difference was statistically significant (P=0.012). Lymph node metastasis of cheek SCCs showed a trend towards a significant association (P= 0.098) with cyclin D1 amplification whereas the lymph node metastasis of tongue SCC was clearly not significant (P=0.593).There was a statistically significant correlation between cyclin D1 positivity and survival rate (P=0.009) for overall SCC cases and (P<0.001) for cheek SCC cases.

CONCLUSION

The present study found that cyclin D1 amplification may differ in different subsites of OSCC (tongue vs cheek) and its positive amplification implies an overall poor survival in OSCCs, particularly those arising in cheeks.

摘要

引言

已经对几种分子标志物作为口腔鳞状细胞癌（OSCC）预后标志物的实用性进行了研究。一种这样的分子标志物是细胞周期蛋白D1，它是人类位于11q13上的原癌基因。

目的

通过荧光原位杂交（FISH）方法探讨将细胞周期蛋白D1用作舌和颊鳞状细胞癌预后标志物的可行性。

方法

从口腔病理诊断实验室档案中获取50个石蜡包埋样本（颊部和舌部鳞状细胞癌各25个）。社会人口统计学数据、组织病理学诊断、淋巴结状态和生存数据来自马来西亚口腔癌数据库和组织库系统（MOCDTBS），该系统由马来亚大学口腔癌研究与协调中心（OCRCC）协调。使用FISH技术按照Vysis方案检测细胞周期蛋白D1的扩增情况。使用Fisher精确检验分析细胞周期蛋白D1与部位和淋巴结状态之间的统计相关性。采用Kaplan-Meier法和对数秩（Mantel-Cox）检验分析细胞周期蛋白D1扩增情况和中位生存时间。

结果

在72%（36个）的OSCC中检测到细胞周期蛋白D1阳性扩增。在舌部肿瘤的88%（22个）和颊部肿瘤的56%（14个）中观察到细胞周期蛋白D1阳性扩增，差异具有统计学意义（P=0.012）。颊部鳞状细胞癌的淋巴结转移与细胞周期蛋白D1扩增有显著关联趋势（P=0.098），而舌部鳞状细胞癌的淋巴结转移明显无显著关联（P=0.593）。总体鳞状细胞癌病例中细胞周期蛋白D1阳性与生存率之间存在统计学显著相关性（P=0.009），颊部鳞状细胞癌病例中则为（P<0.001）。