Kaneko T, Nagata I, Miyamoto S, Kubo H, Kikuchi H, Fujisato T, Ikada Y
Department of Neurosurgery, Kyoto University, Japan.
Stroke. 1992 Nov;23(11):1637-42. doi: 10.1161/01.str.23.11.1637.
The purpose of this study was to assess the effect of nicardipine, a Ca2+ channel blocker, on angiogenesis in vitro.
Bovine carotid artery endothelial cells were cultured between type I collagen gel layers with 10(-9) to 10(-5) M nicardipine. The morphological changes were monitored by phase-contrast microscopy and photographed. The total length of tubular structures was measured with an image analyzer system. Endothelial proliferation and migration assays were also performed with the same doses of nicardipine.
Cultured endothelial cells form tubular structures between collagen gel layers. Tube formation of endothelial cells was suppressed by culture with 10(-9) to 10(-5) M nicardipine in a dose-dependent manner. Migration of endothelial cells was also suppressed by the same doses of nicardipine. However, proliferation of endothelial cells was not enhanced.
Nicardipine acts as an inhibitor of angiogenesis in vitro by inhibiting the migration of endothelial cells. This result suggests that nicardipine may have therapeutic potential in angiogenic disorders such as tumor growth, atherogenesis, and diabetic retinopathy.
本研究旨在评估钙通道阻滞剂尼卡地平在体外对血管生成的影响。
将牛颈动脉内皮细胞培养于I型胶原凝胶层之间，加入10⁻⁹至10⁻⁵M的尼卡地平。通过相差显微镜监测形态变化并拍照。用图像分析系统测量管状结构的总长度。还用相同剂量的尼卡地平进行内皮细胞增殖和迁移试验。
培养的内皮细胞在胶原凝胶层之间形成管状结构。用10⁻⁹至10⁻⁵M的尼卡地平培养可剂量依赖性地抑制内皮细胞的管形成。相同剂量的尼卡地平也抑制内皮细胞的迁移。然而，内皮细胞的增殖未增强。
尼卡地平通过抑制内皮细胞的迁移在体外作为血管生成的抑制剂起作用。该结果表明尼卡地平在诸如肿瘤生长、动脉粥样硬化和糖尿病视网膜病变等血管生成性疾病中可能具有治疗潜力。
