Kaneko T, Nagata I, Kikuchi H, Miyamoto S, Kubo H, Fujisato T, Ikada Y
Department of Neurosurgery, Kyoto University, Japan.
Nihon Geka Hokan. 1992 Mar 1;61(2):150-5.
We studied the effect of nicardipine, a calcium channel blocker, on the morphological change of endothelial cells in vitro. Cultured endothelial cells derived from bovine carotid artery make tubular structures between collagen gel layers. Tube formation of endothelial cells was suppressed by culture with 10(-9)-10(-5) M of nicardipine in a dose dependent manner. Migration of endothelial cells was also suppressed by the same dose of nicardipine. However, proliferation of endothelial cells was not enhanced. These findings suggest that nicardipine acts as an inhibitor of angiogenesis in vitro by inhibiting the migration of endothelial cells.
我们研究了钙通道阻滞剂尼卡地平对体外培养的内皮细胞形态变化的影响。从牛颈动脉分离培养的内皮细胞在胶原凝胶层之间形成管状结构。用10(-9)-10(-5)M的尼卡地平培养时，内皮细胞的管腔形成呈剂量依赖性抑制。相同剂量的尼卡地平也抑制内皮细胞的迁移。然而，内皮细胞的增殖并未增强。这些结果表明，尼卡地平在体外通过抑制内皮细胞的迁移而发挥血管生成抑制剂的作用。
