Lievens Bart, Brouwer Margreet, Vanachter Alfons C R C, Lévesque C André, Cammue Bruno P A, Thomma Bart P H J
Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, 3001, Heverlee-Leuven, Belgium.
FEMS Microbiol Lett. 2003 Jun 6;223(1):113-22. doi: 10.1016/S0378-1097(03)00352-5.
Fusarium wilt, caused by Fusarium oxysporum f. sp. lycopersici, and Verticillium wilt, caused by either Verticillium albo-atrum or Verticillium dahliae, are devastating diseases of tomato (Lycopersicon esculentum) found worldwide. Monitoring is the cornerstone of integrated pest management of any disease. The lack of rapid, accurate, and reliable means by which plant pathogens can be detected and identified is one of the main limitations in integrated disease management. In this paper, we describe the development of a molecular detection system, based on DNA array technology, for rapid and efficient detection of these vascular wilt pathogens. We show the utility of this array for the sensitive detection of these pathogens from complex substrates like soil, plant tissues and irrigation water, and samples that are collected by tomato growers in their greenhouses.
由尖孢镰刀菌番茄专化型（Fusarium oxysporum f. sp. lycopersici）引起的枯萎病，以及由黑白轮枝菌（Verticillium albo-atrum）或大丽轮枝菌（Verticillium dahliae）引起的黄萎病，是在全球范围内发现的番茄（Lycopersicon esculentum）毁灭性病害。监测是任何病害综合虫害管理的基石。缺乏快速、准确和可靠的手段来检测和鉴定植物病原体是综合病害管理的主要限制之一。在本文中，我们描述了一种基于DNA阵列技术的分子检测系统的开发，用于快速有效地检测这些维管束枯萎病原体。我们展示了该阵列在从土壤、植物组织和灌溉水等复杂基质以及番茄种植者在其温室中采集的样本中灵敏检测这些病原体的效用。
