Higher Agronomic Institute of Chott-Mariem, Sousse University, BP.47, Sousse, Tunisia.
Environ Sci Pollut Res Int. 2018 Jul;25(19):18297-18302. doi: 10.1007/s11356-017-0233-6. Epub 2017 Sep 23.
The efficacy of Verticillium leptobactrum isolate (HR1) was evaluated in the control of root-knot nematode and Fusarium wilt fungus under laboratory and greenhouse conditions. Five concentrations of V. leptobactrum (HR1) isolate were tested for their nematicidal and fungicidal activities against Meloidogyne javanica and Fusarium oxysporum f.sp. lycopersici in vitro. Laboratory trials showed that mycelium growth inhibition of Fusarium wilt fungus was correlated to the increase of the concentration of culture filtrate. All dilutions showed efficiency in reducing the growth of Fusarium oxysporum f.sp. lycopersici. The greatest nematicidal activity was observed at 50, 75, and 100% filtrate dilutions. The egg hatching percentage reached 42%, and the juvenile's corrected mortality registered 90% for the above treatments. In greenhouse experiment, the biocontrol agent fungus enhanced significantly tomato growth components (height and weight of plant and root). The multiplication rate of root-knot nematode and the Fusarium wilt disease incidence declined significantly with soil application of V. leptobactrum as with chemical treatments. The isolate HR1 was efficient to control wilt disease complex caused by M. javanica and Fusarium oxysporum f.sp. lycopersici.
采用室内和温室试验，评价淡紫拟青霉（HR1）分离物防治根结线虫和枯萎病菌的效果。在体外测试了 5 种浓度的淡紫拟青霉（HR1）分离物对线虫（南方根结线虫）和枯萎病菌（番茄枯萎病菌）的杀线虫和杀菌活性。室内试验表明，枯萎病菌的菌丝生长抑制与培养液滤液浓度的增加呈正相关。所有稀释度均能有效降低番茄枯萎病菌的生长。在 50%、75%和 100%滤出液稀释度下观察到最大的杀线虫活性。卵孵化率达到 42%，幼虫校正死亡率为 90%。在温室试验中，生防真菌显著增强了番茄生长的各个方面（株高和根重）。与化学处理相比，土壤施用淡紫拟青霉可显著降低根结线虫的繁殖率和枯萎病发病率。HR1 分离物对线虫（南方根结线虫）和枯萎病菌（番茄枯萎病菌）复合病害的防治效果显著。
