发育中小鼠大脑中dbl家族鸟嘌呤核苷酸交换因子的动态协调表达谱。

Dynamic and coordinated expression profile of dbl-family guanine nucleotide exchange factors in the developing mouse brain.

作者信息

Yoshizawa Masato, Sone Masaki, Matsuo Naoki, Nagase Takahiro, Ohara Osamu, Nabeshima Yo-ichi, Hoshino Mikio

机构信息

Department of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, 606-8501 Kyoto, Japan.

出版信息

Gene Expr Patterns. 2003 Jun;3(3):375-81. doi: 10.1016/s1567-133x(03)00002-4.

DOI:10.1016/s1567-133x(03)00002-4

PMID:12799088

Abstract

Dbl-family guanine nucleotide exchange factors (Dbl-GEFs) act as activators of Rho-like small G proteins such as Rac1, Cdc42 and RhoA. Recently, some GEFs have been suggested to play important roles in the development of the nervous system. Here, we report a comprehensive expression profile analysis of 20 Dbl-GEFs that have yet to be well investigated. Northern analyses of murine mRNAs from brains of E13, E17, P7 and adult mice revealed expression of 18 out of 20 GEFs in some or all stages. In addition, we found that three human GEFs were highly expressed in the brain. Examination of the spatial expression patterns of five GEFs in embryos or neonatal brain by in situ hybridization revealed distinct patterns for each GEF. Our study reveals the dynamic and coordinated expression profiles of the Dbl-GEFs and provides a basic framework for understanding the function of GEFs in neural development.

摘要

Dbl家族鸟嘌呤核苷酸交换因子（Dbl-GEFs）作为Rho样小G蛋白（如Rac1、Cdc42和RhoA）的激活剂。最近，一些GEFs被认为在神经系统发育中发挥重要作用。在此，我们报告了对20种尚未得到充分研究的Dbl-GEFs的全面表达谱分析。对来自E13、E17、P7和成年小鼠大脑的鼠类mRNA进行的Northern分析显示，20种GEFs中有18种在某些或所有阶段表达。此外，我们发现三种人类GEFs在大脑中高表达。通过原位杂交检查胚胎或新生大脑中五种GEFs的空间表达模式，发现每种GEF都有独特的模式。我们的研究揭示了Dbl-GEFs的动态和协调表达谱，并为理解GEFs在神经发育中的功能提供了一个基本框架。