Grgic I, Perdih A
Faculty of Chemistry and Chemical Technology, University of Ljubljana, 1000 Ljubljana, Slovenia.
Folia Microbiol (Praha). 2003;48(2):199-202. doi: 10.1007/BF02930956.
Fluorescein derivatives added into the growth medium were decolorized during submerged cultivation of Phanerochaete chrysosporium. The highest decrease of absorbance A450 was observed in the growth phase regardless of the presence of inducers Tween 80 or poly(ethylene glycol) (PEG). Fluorescein linked to PEG was prepared and, after addition to cultures, shown to stimulate the production of lignin peroxidase. Passing of fluorescing substances into hyphae (observed by confocal microscopy) showed that they were concentrated on some structures inside hyphae.
添加到生长培养基中的荧光素衍生物在黄孢原毛平革菌的深层培养过程中发生了脱色。无论是否存在诱导剂吐温80或聚乙二醇（PEG），在生长阶段均观察到吸光度A450的最大降幅。制备了与PEG连接的荧光素，并在添加到培养物后显示出可刺激木质素过氧化物酶的产生。通过共聚焦显微镜观察到荧光物质进入菌丝，表明它们集中在菌丝内的某些结构上。
