Forest Products Laboratory, Forest Service, U. S. Department of Agriculture, Madison, Wisconsin 53705.
Appl Environ Microbiol. 1985 Nov;50(5):1274-8. doi: 10.1128/aem.50.5.1274-1278.1985.
Research on the extracellular hemeprotein ligninases of Phanerochaete chrysosporium has been hampered by the necessity to produce them in stationary culture. This investigation examined the effects of detergents on development of ligninase activity in agitated submerged cultures. Results show that addition of Tween 80, Tween 20, or 3-[(3-colamidopropyl)dimethylammonio]1-propanesulfonate to the cultures permits development of ligninase activity comparable to that routinely obtained in stationary cultures. The detergent-amended cultures express the entire ligninolytic system, assayed as the complete oxidation of [C]lignin to CO(2). The detergent effect is evidently not merely in facilitating release of extant enzyme. Development of ligninolytic activity in the agitated cultures, as in stationary cultures, is idiophasic. Ion-exchange fast protein-liquid chromatography indicated that the heme protein profiles in agitated and stationary cultures are very similar. These findings should make it possible to scale up production of ligninolytic enzymes in stirred tank fermentors.
白腐真菌黄孢原毛平革菌胞外血红素木素酶的研究一直受到必须在静置培养中生产它们的阻碍。本研究考察了洗涤剂对搅拌浸没培养中木素酶活性发展的影响。结果表明，向培养物中添加吐温 80、吐温 20 或 3-[(3-酰胺丙基)二甲基氨基]丙磺酸钠允许发展与在静置培养中常规获得的木素酶活性相当的木素酶活性。用洗涤剂处理的培养物表达整个木质素分解系统，如用[C]木质素完全氧化为 CO(2)来测定。显然，洗涤剂的作用不仅仅是促进现有酶的释放。在搅拌培养物中，与在静置培养物中一样，木质素分解活性的发展是同型的。离子交换快速蛋白质液相色谱表明，搅拌和静置培养物中的血红素蛋白图谱非常相似。这些发现应该使在搅拌罐发酵罐中扩大木质素分解酶的生产成为可能。
