α1（I）前胶原基因在α1（I）缺乏的、化学转化的叙利亚仓鼠胚胎成纤维细胞中的转录后调控

Post-transcriptional regulation of the pro alpha 1(I) collagen gene in pro alpha 1(I)-deficient, chemically transformed Syrian hamster embryo fibroblasts.

作者信息

Schalk E M, Gosiewska A, Prather W, Peterkofsky B

机构信息

Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.

出版信息

Biochem Biophys Res Commun. 1992 Oct 30;188(2):780-5. doi: 10.1016/0006-291x(92)91124-9.

DOI:10.1016/0006-291x(92)91124-9

PMID:1280122

Abstract

4-Nitroquinoline-1-oxide-transformed Syrian hamster embryo fibroblasts (NQT-SHE) synthesize the pro alpha 2 chain but not the pro alpha 1 subunit of type I procollagen, and they contain little pro alpha 1(I)mRNA. This study shows that there was no accumulation of pro alpha 1(I) poly(A)+ mRNA in NQT-SHE fibroblasts. BHK cells, a normal established line of hamster fibroblasts that synthesized collagen at approximately the same rate as NQT-SHE fibroblasts, nevertheless produced both subunits of type I collagen and contained pro alpha 1(I)mRNA. Run-off transcription assays with isolated nuclei showed that both the pro alpha 1(I) and pro alpha 2(I) genes were transcribed at about the same rate in NQT-SHE cells as well as in the normal BHK cells. These results suggest that a post-transcriptional defect, probably resulting from transformation, prevents the accumulation of pro alpha 1(I)mRNA in NQT-SHE cells.

摘要

4-硝基喹啉-1-氧化物转化的叙利亚仓鼠胚胎成纤维细胞（NQT-SHE）能合成I型前胶原的前α2链，但不能合成前α1亚基，并且它们含有的前α1（I）mRNA很少。本研究表明，NQT-SHE成纤维细胞中不存在前α1（I）多聚腺苷酸加尾mRNA的积累。BHK细胞是仓鼠成纤维细胞的正常细胞系，其合成胶原蛋白的速率与NQT-SHE成纤维细胞大致相同，但仍能产生I型胶原蛋白的两个亚基，并含有前α1（I）mRNA。用分离的细胞核进行的连续转录分析表明，前α1（I）和前α2（I）基因在NQT-SHE细胞以及正常BHK细胞中的转录速率大致相同。这些结果表明，可能由转化导致的转录后缺陷阻止了前α1（I）mRNA在NQT-SHE细胞中的积累。