Kähäri V M, Multimäki P, Vuorio E
FEBS Lett. 1987 May 11;215(2):331-4. doi: 10.1016/0014-5793(87)80172-2.
Fibroblasts cultured from affected and unaffected skin sites of three scleroderma patients were studied for the activation of type I collagen gene expression. Dot blot hybridizations with pro alpha 2(I) collagen specific cDNA probe revealed 2.9-4.8-fold increases in pro alpha 2(I) mRNA levels in the affected fibroblasts over the unaffected control cells. Transcription rate of the pro alpha 2(I) gene in the nuclei isolated from the same cells was 2.0-3.7-fold higher in the scleroderma fibroblasts than in the controls. The results show that scleroderma fibroblasts have undergone activation of collagen gene expression at the transcriptional level, which subsequently results in elevated procollagen mRNA levels, overproduction of collagen, and development of dermal fibrosis.
对三名硬皮病患者患病和未患病皮肤部位培养的成纤维细胞进行了研究，以检测I型胶原蛋白基因表达的激活情况。用原α2（I）胶原蛋白特异性cDNA探针进行斑点印迹杂交分析显示，与未受影响的对照细胞相比，患病成纤维细胞中的原α2（I）mRNA水平增加了2.9至4.8倍。从相同细胞中分离出的细胞核中，硬皮病成纤维细胞的原α2（I）基因转录率比对照高2.0至3.7倍。结果表明，硬皮病成纤维细胞在转录水平上经历了胶原蛋白基因表达的激活，这随后导致前胶原蛋白mRNA水平升高、胶原蛋白过度产生以及皮肤纤维化的发展。
