Pologruto Thomas A, Sabatini Bernardo L, Svoboda Karel
Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
Biomed Eng Online. 2003 May 17;2:13. doi: 10.1186/1475-925X-2-13.
Laser scanning microscopy is a powerful tool for analyzing the structure and function of biological specimens. Although numerous commercial laser scanning microscopes exist, some of the more interesting and challenging applications demand custom design. A major impediment to custom design is the difficulty of building custom data acquisition hardware and writing the complex software required to run the laser scanning microscope.
We describe a simple, software-based approach to operating a laser scanning microscope without the need for custom data acquisition hardware. Data acquisition and control of laser scanning are achieved through standard data acquisition boards. The entire burden of signal integration and image processing is placed on the CPU of the computer. We quantitate the effectiveness of our data acquisition and signal conditioning algorithm under a variety of conditions. We implement our approach in an open source software package (ScanImage) and describe its functionality.
We present ScanImage, software to run a flexible laser scanning microscope that allows easy custom design.
激光扫描显微镜是分析生物样本结构和功能的强大工具。尽管存在众多商用激光扫描显微镜，但一些更有趣且具挑战性的应用需要定制设计。定制设计的一个主要障碍是构建定制数据采集硬件以及编写运行激光扫描显微镜所需的复杂软件存在困难。
我们描述了一种基于软件的简单方法，无需定制数据采集硬件即可操作激光扫描显微镜。通过标准数据采集板实现数据采集和激光扫描控制。信号积分和图像处理的全部负担都由计算机的中央处理器承担。我们在各种条件下对数据采集和信号调节算法的有效性进行了量化。我们在一个开源软件包（ScanImage）中实现了我们的方法并描述了其功能。
我们展示了ScanImage，这是一款运行灵活的激光扫描显微镜的软件，便于进行定制设计。
