Kirshenbaum Arnold S, Akin Cem, Wu Yalin, Rottem Menachem, Goff Julie P, Beaven Michael A, Rao V Koneti, Metcalfe Dean D
Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-1881, USA.
Leuk Res. 2003 Aug;27(8):677-82. doi: 10.1016/s0145-2126(02)00343-0.
Two novel stem cell factor (SCF) dependent human mast cell lines, designated LAD 1 and 2, were established from bone marrow aspirates from a patient with mast cell sarcoma/leukemia. LAD 1 and 2 cells have the ultrastructural features of human mast cells, and express FcepsilonRI, CD4, 9, 13, 14, 22, 31, 32, 45, 64, 71, 103, 117, 132, CXCR4 (CD184), CCR5 (CD195); and intracytoplasmic histamine, tryptase and chymase. LAD 1 and 2 do not exhibit activating mutations at codon 816 of c-kit. Both LAD 1 and 2 release beta-hexosaminidase following FcepsilonRI or FcgammaRI aggregation. The availability of these cell lines offers an unparalleled circumstance to examine the biology of human mast cells.
从一名肥大细胞肉瘤/白血病患者的骨髓抽吸物中建立了两种新的依赖干细胞因子（SCF）的人肥大细胞系，命名为LAD 1和LAD 2。LAD 1和LAD 2细胞具有人肥大细胞的超微结构特征，并表达FcepsilonRI、CD4、9、13、14、22、31、32、45、64、71、103、117、132、CXCR4（CD184）、CCR5（CD195）；以及细胞质内的组胺、类胰蛋白酶和糜蛋白酶。LAD 1和LAD 2在c-kit的816密码子处未表现出激活突变。LAD 1和LAD 2在FcepsilonRI或FcgammaRI聚集后均释放β-己糖胺酶。这些细胞系的可用性为研究人肥大细胞生物学提供了无与伦比的条件。
